In vitro assessment of the cytotoxic and mutagenic potential of perfluorooctanoic acid
In vitro assessment of the cytotoxic and mutagenic potential of perfluorooctanoic acid
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DOI：
10.1016/j.tiv.2008.04.004
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发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Hazen, M. J.
中科院分区：
文献类型：
--
作者：
Fernandez Freire, P.;Martin, J. M. Perez;Hazen, M. J.
全氟辛酸（PFOA）是一种全氟化合物，广泛存在于环境中，包括野生动物和人类。尽管有可用的信息，但关于PFOA在非肿瘤哺乳动物细胞中的细胞毒性的研究相对有限。在这项工作中，两个体外毒性系统，以提供进一步了解PFOA的细胞毒性和致突变潜力。采用生物化学和形态学参数评价化学品对Vero细胞的细胞毒性，同时根据艾姆斯试验评价致突变性。高剂量的PFOA会引起Vero细胞的氧化应激，这与细胞周期停滞在G1期和诱导细胞凋亡密切相关。我们的研究结果证实了以前在人类肿瘤细胞中的研究结果，并表明这种全氟化合物的作用方式在哺乳动物细胞类型中并不特殊。另一方面，在加或不加大鼠S9代谢活化系统的情况下，使用4种鼠伤寒沙门氏菌菌株进行的艾姆斯试验中，该化合物无致突变性。(C)2008爱思唯尔有限公司保留所有权利。
Perfluorooctanoic acid (PFOA) is a perfluorinated compound ubiquitously detected in the environment, including wildlife and humans. Despite the available information, research on the cytotoxicity of PFOA in non-tumoral mammalian cells is relatively limited. In this work, two in vitro toxicity systems were employed to provide further insight into the cytotoxic and mutagenic potential of PFOA. The cytotoxicity of the chemical towards Vero cells was assessed using biochemical and morphological parameters, while mutagenicity was evaluated according to Ames test. High doses of PFOA cause oxidative stress in Vero cells, that was closely linked to cell cycle arrest at the G1 phase and induction of apoptosis. Our results corroborate previous findings in human tumoral cells and suggest that the mode of action of this perfluorinated compound is not a peculiarity among mammalian cell types. On the other hand, the compound was not mutagenic in the Ames test, using four strains of Salmonella typhimurium in the presence or absence of rat S9 metabolic activation system. (C) 2008 Elsevier Ltd. All rights reserved.