Osteogenic differentiation of human placenta-derived mesenchymal stem cells (PMSCs) on electrospun nanofiber meshes
Osteogenic differentiation of human placenta-derived mesenchymal stem cells (PMSCs) on electrospun nanofiber meshes
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DOI：
10.1007/s10616-012-9450-5
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发表时间：
2012-04
期刊：
影响因子：
2.2
通讯作者：
Dongmei Zhang;A. Tong;Liangxue Zhou;F. Fang;G. Guo
中科院分区：
文献类型：
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作者：
Dongmei Zhang;A. Tong;Liangxue Zhou;F. Fang;G. Guo
在成功地将基于细胞的骨组织修复治疗研究转化为临床研究方面仍然存在许多挑战，包括合适的细胞源和可行的细胞输送系统。聚乙二醇-聚己内酯(ε-PCL)两亲嵌段共聚物在微球制备中得到了广泛的探索。由于聚乙二醇亲水链段被引入到聚己内酯的骨架中，这些共聚物在携带蛋白质、亲脂药物或基因方面显示出比常用的聚己内酯(ε)和聚乳酸更大的潜力。本研究旨在研究人胎盘来源的间充质干细胞(PMSCs)在聚乙二醇-聚己内酯三嵌段共聚物纳米纤维支架上的附着和成骨分化情况。在这里，我们证明了PMSCs可以在纳米纤维支架上旺盛地增殖，并能有效地分化为成骨细胞。本研究为纳米纤维支架作为PMSCs体外培养的理想支持材料和体内骨修复细胞输送载体的应用提供了证据。
Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in microspheres preparation. Due to the introduction of hydrophilic PEG segments into PCL backbones, these copolymers have shown much more potentials in carrying protein, lipophilic drugs or genes than commonly used poly (ε-caprolactone) (PCL) and poly (lactic acid). The aim of this study is to investigate the attachment and osteogenic differentiation of human placenta derived mesenchymal stem cells (PMSCs) on PEG-PCL triblock copolymers nanofiber scaffolds. Here we demonstrated that PMSCs proliferate robustly and can be effectively differentiated into osteogenic-like cells on nanofiber scaffolds. This study provides evidence for the use of nanofiber scaffolds as an ideal supporting material for in vitro PMSCs culture and an in vivo cell delivery vehicle for bone repair.