Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure
Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure
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DOI：
10.1002/pld3.137
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发表时间：
2019-05-01
期刊：
影响因子：
3
通讯作者：
Shimazaki, Ken-ichiro
中科院分区：
文献类型：
--
作者：
Iwai, Sumio;Ogata, Sho;Shimazaki, Ken-ichiro
活性氧（Reactive oxygen species，ROS）是一种普遍存在的信号分子，参与气孔关闭等多种生理过程。光合电子传递（PET）是植物产生ROS的主要来源，但它是否在保卫细胞信号转导中起作用尚不清楚。在这里，我们评估了PET是否在保卫细胞中的脱落酸（阿坝）信号传导中起作用。ABA诱导的活性氧在光照下定位于拟南芥、鸭跖草和蚕豆的保卫细胞叶绿体中，并被PET抑制剂3-（3，4-二氯苯基）-1，1-二甲基脲和2，5-二溴-3-甲基-6-异丙基对苯醌所消除。这些抑制剂减少ABA诱导的气孔关闭在所有三个物种，以及在NADPH氧化酶缺乏突变体atrboh D/F。然而，NADPH氧化酶抑制剂不能完全消除ABA诱导的叶绿体中的ROS，并且在atrboh D/F的保卫细胞叶绿体中仍然观察到ABA诱导的ROS。这项研究表明，通过PET产生的活性氧作为信号分子在ABA诱导的气孔关闭，这发生在音乐会与来自NADPH氧化酶的活性氧。
Reactive oxygen species (ROS) are ubiquitous signaling molecules involved in diverse physiological processes, including stomatal closure. Photosynthetic electron transport (PET) is the main source of ROS generation in plants, but whether it functions in guard cell signaling remains unclear. Here, we assessed whether PET functions in abscisic acid (ABA) signaling in guard cells. ABA-elicited ROS were localized to guard cell chloroplasts in Arabidopsis thaliana, Commelina benghalensis, and Vicia faba in the light and abolished by the PET inhibitors 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea and 2, 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone. These inhibitors reduced ABA-induced stomatal closure in all three species, as well as in the NADPH oxidase-lacking mutant atrboh D/F. However, an NADPH oxidase inhibitor did not fully eliminate ABA-induced ROS in the chloroplasts, and ABA-induced ROS were still observed in the guard cell chloroplasts of atrboh D/F. This study demonstrates that ROS generated through PET act as signaling molecules in ABA-induced stomatal closure and that this occurs in concert with ROS derived through NADPH oxidase.