OsELF3 is involved in circadian clock regulation for promoting flowering under long-day conditions in rice.
OsELF3 is involved in circadian clock regulation for promoting flowering under long-day conditions in rice.
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DOI：
10.1093/mp/sss062
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发表时间：
2013
期刊：
影响因子：
27.5
通讯作者：
Ying Yang;Qiang Peng;Guoxing Chen;Xianghua Li;Changyin Wu
中科院分区：
文献类型：
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作者：
Ying Yang;Qiang Peng;Guoxing Chen;Xianghua Li;Changyin Wu
抽穗期是决定水稻种植季节和区域适应性的重要性状。在过去的十年中，研究工作已经确定了一些重要的光周期途径基因是保守的拟南芥和水稻之间。在这项研究中，我们确定了一个新的基因，水稻ELF3（OsELF3），这是一个假定的同源物的ELF3基因在拟南芥。OsELF3是长日照条件下控制抽穗期所必需的基因。其Tos17标记突变体仅在长日照条件下表现出延迟抽穗表型，而在短日照条件下不表现出延迟抽穗表型。OsELF3蛋白在叶片中高表达，定位于核仁中。OsELF3转录水平的日变化具有明显的节律性，在野生型植株中，OsELF3转录水平在白天出现低谷，在深夜出现高峰。然而，这种表达模式在oself3突变体中被破坏。进一步的研究表明OsGI和Ghd7在oself3突变体中的表达上调，表明OsELF3在长日照条件下对开花时间的调控中起着OsGI和Ghd7上游的负调控作用。在oself3突变体中，生物钟相关基因（包括OsPRR成员）的节律性表达受到明显影响。我们的研究结果表明，OsELF3作为一个花激活剂在长日照光周期途径通过其串扰与昼夜节律钟在水稻。
Heading date is a critical trait that determines cropping seasons and regional adaptability in rice (Oryza sativa). Research efforts during the last decade have identified some important photoperiod pathway genes that are conserved between Arabidopsis and rice. In this study, we identified a novel gene, Oryza sativa ELF3 (OsELF3), which is a putative homolog of the ELF3 gene in Arabidopsis thaliana. OsELF3 was required for the control of heading date under long-day conditions. Its Tos17-tagging mutants exhibited a delayed heading date phenotype only under long-day, but not short-day, conditions. OsELF3 was highly expressed in leaf blades, and the OsELF3 protein was localized in the nucleolus. An obvious diurnal rhythm of OsELF3 transcript level was observed, with a trough in the early day and a peak in the late night in wild-type plants. However, this expression pattern was disrupted in oself3 mutants. Further investigations showed that the expression of OsGI and Ghd7 was up-regulated in the oself3 mutant, indicating that OsELF3 acts as a negative regulator upstream of OsGI and Ghd7 in the flowering-time control under long-day conditions. The rhythmic expression of circadian clock-related genes, including some OsPRR members, was obviously affected in oself3 mutants. Our results indicated that OsELF3 acts as a floral activator in the long-day photoperiodic pathway via its crosstalk with the circadian clock in rice.