Quenching Activities of Common Hydrophilic and Lipophilic Antioxidants against Singlet Oxygen Using Chemiluminescence Detection System
Quenching Activities of Common Hydrophilic and Lipophilic Antioxidants against Singlet Oxygen Using Chemiluminescence Detection System
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使用化学发光检测系统测定常见亲水性和亲脂性抗氧化剂对单线态氧的猝灭活性
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
E. Yamashita
中科院分区：
文献类型：
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作者：
Y. Nishida;E. Yamashita
在相同的测试条件下记录了常见的亲水性和亲脂性抗氧化剂如多酚、生育酚、类胡萝卜素、抗坏血酸、辅酶Q10和α-硫辛酸的单线态氧猝灭活性：化学发光检测系统使用1,4-二甲基萘的热解离内过氧化物作为O2发生器，在DMF：CDCl3（9：1）中进行直接O2计数。类胡萝卜素比其他抗氧化剂表现出更大的总猝灭速率常数，其中虾青素表现出最强的活性。 α-生育酚和α-硫辛酸表现出相当大的活性，而抗坏血酸、CoQ10和多酚的活性则很弱；其中包括辣椒素、普罗布考、依达拉文、BHT 和 Trolox。该系统有潜力成为评估各种亲水性和亲脂性化合物对单线态氧的猝灭活性的强大工具。
The singlet oxygen quenching activities among common hydrophilic and lipophilic antioxidants such as polyphenols, tocopherols, carotenoids, ascorbic acid, coenzyme Q10 and α-lipoic acid were recorded under the same test condition: the chemiluminescence detection system for direct O2 counting using the thermodissociable endoperoxides of 1,4-dimethylnaphthalene as O2 generator in DMF : CDCl3 (9 : 1). Carotenoids exhibited larger total quenching rate constants than other antioxidants, with astaxanthin showing the strongest activity. α-Tocopherol and α-lipoic acid showed considerable activities, whereas the activities of ascorbic acid, CoQ10 and polyphenols were only slight; these included capsaicin, probucol, edaravon, BHT and Trolox. This system has the potential of being a powerful tool to evaluate the quenching activity against singlet oxygen for various hydrophilic and lipophilic compounds.