Microaerobic dark fermentative hydrogen production by the photosynthetic bacterium, Rhodobacter capsulatus JP91
Microaerobic dark fermentative hydrogen production by the photosynthetic bacterium, Rhodobacter capsulatus JP91
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DOI：
10.1093/ijlct/cts011
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发表时间：
2012-06-01
影响因子：
2.3
通讯作者：
Hallenbeck, Patrick C.
中科院分区：
文献类型：
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作者：
Abo-Hashesh, Mona;Hallenbeck, Patrick C.
光合细菌Rhodobacter capsulatus在氮限制的厌氧光合条件下产生氢气。本研究探讨了R.在微氧条件下，在黑暗中，在有限量的O-2和固定氮的情况下，capsulatus可以产生氢气。以一株产氢菌Hup为试材，研究了产氢量与不同O-2浓度、碳源及两种不同N源（谷氨酸和铵）的关系。囊状的在R.的暗分批培养中考察了不同O2-浓度（范围为0.5%至20%）对产氢的影响。在RCV培养基上生长。以各种浓度（20-40 mM）使用不同的碳源，例如葡萄糖、琥珀酸盐、乳酸盐、乙酸盐和苹果酸盐。类似地，检查不同浓度的谷氨酸盐和铵（2- 9mM）的最佳微氧暗氢产生。在4- 8%的O-2浓度下观察到最大的氢气产生。O-2浓度与生长呈显著正相关（r（2）= 0.67），与产氢率呈显著负相关（r（2）= 20.3）。琥珀酸盐（25 mM）与谷氨酸盐（3.5 mM）一起产生最高的比氢生产率[5.61 mmol氢/（mg细胞干重/ml）]。最大平均氢产率为0.6 mol氢/mol苹果酸，随后为0.41 mol氢/mol乳酸，0.36 mol氢/mol琥珀酸，而最小量的氢由葡萄糖和乙酸产生（分别为0.16 mol氢/mol和0.07 mol氢/mol）。开发一个系统，能够提高产氢的影响进行了讨论。
The photosynthetic bacterium Rhodobacter capsulatus produces hydrogen under nitrogen-limited, anaerobic, photosynthetic conditions. The present study examined whether R. capsulatus can produce hydrogen under microaerobic conditions in the dark with limiting amounts of O-2 and fixed nitrogen. The relationship between hydrogen production, different O-2 concentrations and carbon sources as well as two different N sources, glutamate and ammonium, were studied in batch culture using a Hup strain of R. capsulatus. The effect of different O-2 concentrations, ranging from 0.5 to 20%, on hydrogen production was examined in dark batch cultures of R. capsulatus grown on RCV medium. Different carbon sources, e.g. glucose, succinate, lactate, acetate and malate, were used at various concentrations (20-40 mM). Similarly, different concentrations of glutamate and ammonium (2-9 mM) were examined for optimum microaerobic dark hydrogen production. Maximum hydrogen production was observed at an O-2 concentration of 4-8%. There was a highly positive correlation between O-2 and growth (r(2) = 0.67), whereas O-2 concentration and hydrogen productivity were negatively correlated (r(2) = 20.3). Succinate (25 mM) together with glutamate (3.5 mM) gave the highest specific hydrogen productivity [5.61 mmol hydrogen/(mg cell dry weight/ml)]. The maximum average hydrogen yield was 0.6 mol hydrogen/mol malate followed by 0.41 mol hydrogen/mol lactate, 0.36 mol hydrogen/mol succinate, whereas minimum amounts of hydrogen were produced from glucose and acetate (0.16 mol hydrogen/mol and 0.07 mol hydrogen/mol, respectively). The implications for developing a system capable of improved hydrogen production are discussed.