The colorimetric determination of lactic acid in biological material.
The colorimetric determination of lactic acid in biological material.
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DOI：
10.1016/s0021-9258(18)51379-x
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发表时间：
1941-04
影响因子：
4.8
通讯作者：
S. B. Barker;W. H. Summerson
中科院分区：
文献类型：
--
作者：
S. B. Barker;W. H. Summerson
用浓H2SO 4将乳酸转化为乙醛，并通过其在铜离子存在下与对羟基联苯的显色反应来估计乙醛的量。通过光电色度计测量颜色，使用峰值透射率为560 mµ的滤光片。0.1 μg的差异。乳酸/100 ml。是可以检测到的对于各种材料，所获得的结果与通过Friedemannet等人的滴定法（J. Biol. Chem.，1927，73，335）。研究的大多数物质并不干扰估计，但少数化合物会干扰估计。J·达克沃斯
The lactic acid was converted to acetaldehyde by means of concentrated H2S04and the quantity of this estimated by its colour reaction withp-hydroxydiphenyl in the presence of cupric ions. The colour was measured by photo-electric colorimeter, a filter with peak transmission at 560 mµ being used. Differences of 0.1 µg. lactic acid per 100 ml. were detectable. The results obtained agreed well, for a variety of materials, with those obtained by the titrimetric method of Friedemannet al(J. Biol. Chem., 1927, 73, 335). Most of the substances studied did not interfere with the estimation but a few compounds did.-J. Duckworth.