HPLC Separation of Enantiomers of Some Flavanone Derivatives Using Polysaccharide-Based Chiral Selectors Covalently Immobilized on Silica
HPLC Separation of Enantiomers of Some Flavanone Derivatives Using Polysaccharide-Based Chiral Selectors Covalently Immobilized on Silica
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DOI：
10.1007/s10337-015-3014-8
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发表时间：
2016-02-01
期刊：
影响因子：
1.7
通讯作者：
Chankvetadze, Bezhan
中科院分区：
文献类型：
--
作者：
Fanali, Chiara;Fanali, Salvatore;Chankvetadze, Bezhan
The separation of enantiomers of flavanone and 5 chiral flavanone derivatives was studied on two experimental polysaccharide-based chiral columns by high-performance liquid chromatography with methanol, ethanol, acetonitrile, n-hexane/ethanol and n-hexane/2-propanol as mobile phases with emphasis on the effect of analyte and chiral selector structure on separation parameters. Since chiral selectors were covalently immobilized onto the surface of silica, it was possible to use a wider variety of mobile phases in order to optimize enantioseparations. Together with the backbone of the polysaccharide, the significant role of substituents in the phenyl moiety of chiral selectors, as well as the structure of the analyte was observed on retention and enantioselectivity.
