Association of hypoadiponectinemia with smoking habit in men
Association of hypoadiponectinemia with smoking habit in men
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DOI：
10.1161/01.hyp.0000169444.05588.4c
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发表时间：
2005-06-01
期刊：
影响因子：
8.3
通讯作者：
Ogihara, T
中科院分区：
文献类型：
--
作者：
Iwashima, Y;Katsuya, T;Ogihara, T
脂联素是肥胖、代谢综合征和心血管疾病的重要分子。另一方面，众所周知吸烟习惯与心血管疾病和高血压有关。为了研究脂联素浓度与吸烟习惯之间的关系，我们进行了流行病学调查和人体急性暴露试验以及脂肪细胞实验，以阐明脂联素与吸烟之间相关性的机制。在流行病学研究中，我们共招募了331名男性受试者来检查慢性吸烟暴露。目前吸烟者的血浆脂联素水平（5.3 +/- 0.3 μ g/mL）显著低于从不吸烟者（6.5 +/- 0.4 μ g/mL）（P = 0.01）。在包括年龄、体重指数、高血压、糖尿病、高脂血症和肌酐清除率的多元回归分析中也证实了吸烟和低脂联素水平之间的显著关联（从不吸烟者6.5 +/- 0.4 μ g/mL;过去吸烟者5.6 +/- 0.3 μ g/mL;当前吸烟者5.2 +/- 0.4 μ g/mL; F = 4.52; P = 0.01）。为了研究吸烟对脂联素浓度的急性影响，我们测定了5名从不吸烟的男性在吸烟前和吸烟后3、6和12小时的血浆脂联素水平，结果表明，吸烟后脂联素水平显著下降（12小时;-14.5 +/-0.6%; P < 0.01）。在培养的小鼠3 T3-L1脂肪细胞中，H2 O2和尼古丁以剂量依赖的方式降低脂联素的mRNA表达和分泌。吸烟习惯与男性脂联素浓度相关，其抑制作用部分通过吸烟直接抑制脂肪细胞中脂联素的表达来介导。
Adiponectin is emerging as an important molecule in obesity, the metabolic syndrome, and cardiovascular disease. On the other hand, smoking habit is well known to be related to cardiovascular disease and hypertension. To examine the association between adiponectin concentration and smoking habit, we performed an epidemiological survey and an acute exposure test in humans and an experiment in adipocytes to elucidate the mechanism underlying the association between adiponectin and smoking. In the epidemiological study, we enrolled a total of 331 male subjects to examine chronic smoking exposure. Plasma adiponectin was significantly lower ( P = 0.01) in current smokers ( 5.3 +/- 0.3 mu g/mL) than in never-smokers (6.5 +/- 0.4 mu g/mL). A significant association between smoking and low adiponectin level was also confirmed in multiple regression analysis including age, body mass index, hypertension, diabetes, hyperlipidemia, and creatinine clearance ( never- smokers 6.5 +/- 0.4 mu g/mL; past smokers 5.6 +/- 0.3 mu g/mL; current smokers 5.2 +/- 0.4 mu g/mL; F = 4.52; P = 0.01). To examine the acute effect of smoking on adiponectin concentration for 12 hours, we measured plasma adiponectin level in 5 male never- smokers before smoking and 3, 6, and 12 hours after smoking, with the result that adiponectin showed a significant decrease after smoking ( 12 hours; - 14.5 +/- 0.6%; P < 0.01). In cultured mouse 3T3-L1 adipocytes, H2O2 and nicotine reduced the mRNA expression and secretion of adiponectin in a dose-dependent manner. Smoking habit is associated with adiponectin concentration in men, and its suppressive effect is mediated in part through direct inhibition of smoking on adiponectin expression in adipocytes.