Development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains
Development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains
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DOI：
10.1007/s00203-017-1357-2
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发表时间：
2017-08-01
影响因子：
2.8
通讯作者：
Reis, Veronica Massena
中科院分区：
文献类型：
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作者：
dos Santos, Silvana Gomes;Ribeiro, Flaviane da Silva;Reis, Veronica Massena
固氮菌能够刺激植物生长。本研究旨在评价接种5个固氮菌对甘蔗的促生长作用和硝酸还原酶（NR，1.7.1.1）活性的影响。从三个培养阶段进行了实验：发芽，试管，和在水培。在前两个阶段，采用了7个处理：未接种对照; 5个菌株混合接种;单独接种葡萄糖酸醋杆菌重氮营养型（Gd），Herbaspirillum rubrisubalbicans（Hr），Herbaspirillum seropedicae（Hs），Nitrobellum amazonense（Na），和Paraburkholderia tropica（Pt）。将表现出最佳性能的四个处理转移到水培系统中以分析NR活性。HS、Pt和所有菌株的混合物导致试管中的幼苗生物量最高，其次是Hr。在水培中，混合物和菌株Hr具有最高的促生长效果。NR活性的影响接种只有在低氮供应条件下，与Hr，铂，和混合物的积极影响。
Diazotrophs are able to stimulate plant growth. This study aimed at evaluating the effect of inoculation of five diazotrophic strains on growth promotion and nitrate reductase (NR, EC 1.7.1.1) activity in sugarcane. An experiment was carried out from three stages of cultivation: sprouting, tubes, and in hydroponics. On the first two stages, seven treatments were adopted: uninoculated control; mixed inoculation with five strains; and individual inoculation with Gluconacetobacter diazotrophicus (Gd), Herbaspirillum rubrisubalbicans (Hr), Herbaspirillum seropedicae (Hs), Nitrospirillum amazonense (Na), and Paraburkholderia tropica (Pt). The four treatments showing the best performance were transferred to the hydroponic system for analysis of NR activity. Hs, Pt, and the mixture of all strains led to the highest seedling biomass in tubes, followed by Hr. In hydroponics, the mixture and the strain Hr had the highest growth-promoting effect. NR activity was influenced by inoculation only under low N supply conditions, with positive effect of Hr, Pt, and the mixture.