ALLOANTIGENS DERIVED FROM STIMULATOR CELLS AND BOUND ONTO MLC-ACTIVATED RAT T-LYMPHOBLASTS
ALLOANTIGENS DERIVED FROM STIMULATOR CELLS AND BOUND ONTO MLC-ACTIVATED RAT T-LYMPHOBLASTS
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DOI：
10.1111/j.1365-3083.1979.tb03284.x
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发表时间：
1979-01-01
影响因子：
3.7
通讯作者：
WIGZELL, H
中科院分区：
文献类型：
--
作者：
FENNER, M;FRISCHKNECHT, H;WIGZELL, H
大鼠 T [胸腺来源] 淋巴细胞在 MLC（混合白细胞培养物）中针对同种异体刺激细胞被激活。当使用 T 和 B [骨髓来源] 淋巴细胞的混合物作为应答细胞时，少数百分比的此类 T 母细胞表面携带 Ig[免疫球蛋白]M。当纯化的 T 淋巴细胞作为应答者时，Ig 不存在。大部分 MLC T 淋巴母细胞表面携带刺激物同种抗原。使用抗刺激同种抗体和 FITC（异硫氰酸荧光素异构体 1）标记的兔抗大鼠 Ig 的 F(ab)2 片段进行间接免疫荧光，并通过刺激细胞内部标记后的放射自显影来证明这些抗原，这表明标记从刺激细胞转移到应答细胞。对于生化分析，用内部标记的同种异体或同种刺激细胞重新刺激T母细胞，并在SDS-PAGE（十二烷基硫酸钠-聚丙烯酰胺凝胶电泳）上分析结合的放射性标记的刺激同种异体抗原。在 MLC T 母细胞上发现了分子量为 45,000 道尔顿的完整 SD [血清学定义]抗原和来自刺激细胞的 LD [淋巴细胞定义]决定簇，其分子量为 34,000 道尔顿。没有发现 27,000 道尔顿 Ia 链与 T 爆炸结合的证据。讨论了这些分子在响应者爆炸中的可能排列。
Rat T [thymus-derived] lymphocytes were activated in MLC (mixed leukocyte culture) against allogeneic stimulator cells. A few percent of such T blasts were shown to carry Ig[immunoglobulin]M on their surface when a mixture of T and B [bone marrow-derived] lymphocytes was used as responder cells. The Ig was absent when purified T lymphocytes served as responders. A large proportion of MLC T lymphoblasts carried stimulator alloantigens on their surface. These antigens were demonstrated by indirect immunofluorescence using anti-stimulator alloantibody and FITC (fluorescein isothiocyanate Isomer 1)-labeled F(ab)2 fragments of rabbit anti-rat Ig and by autoradiography after internal labeling of stimulator cells, which showed the transfer of label from stimulator to responder cells. For biochemical analysis T blasts were re-stimulated with internally labeled allogeneic or syngeneic stimulator cells and bound radiolabeled stimulator alloantigens were analyzed on SDS-PAGE (sodium lauryl sulfate-polyacrylamide gel electrophoresis). Intact SD [serologically defined] antigens with MW of 45,000 daltons and LD [lymphocyte-defined] determinants with a MW of 34,000 daltons derived from stimulator cells are found on MLC T blasts. No evidence of binding of the 27,000-dalton Ia chain to T blasts was found. Possible arrangements of these molecules on the responder blasts is discussed.