Hexabrachion proteins in embryonic chicken tissues and human tumors.
Hexabrachion proteins in embryonic chicken tissues and human tumors.
复制标题
胚胎鸡组织和人类肿瘤中的六边形蛋白。
DOI：
10.1083/jcb.105.3.1387
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发表时间：
1987-09
影响因子：
7.8
通讯作者：
Taylor, H C
中科院分区：
文献类型：
--
作者：
Erickson, H P;Taylor, H C
鸡胚和人成纤维细胞的细胞培养物产生具有六臂结构的大细胞外基质分子，我们称之为六臂体（Erickson，H. P.，和J.L.等。Iglesias，1984，Nature（Lond.），311：267-269。在目前的工作中，我们已经确定，由M. Chiquet和D. M.在鸡组织中的Fambrough（1984，J. Cell Biol.，98：1926-1936），以及Bourdon等人在人肿瘤中描述的神经胶质瘤间充质细胞外基质蛋白（Bourdon，M.一、C. J. Wikstrand，H. Furthmayr，T.马修斯和D. D. Bigner，1983，Cancer Res.43：2796-2805）具有六臂体的结构。我们还表明，M1抗原存在于胚胎脑，它是以前报道的缺席，并已纯化的hexabrachion从脑匀浆。最近描述的细胞生长素（Grumet，M.，S.霍夫曼，K. L. Crossin和G. M. Edelman，1985，Proc. Natl. Acad. Sci. USA，82：8075-8079）现在似乎与鸡六臂体蛋白相同。在寻找功能作用的过程中，我们寻找了可能的细胞附着活性。一个强大的，纤连蛋白样的附着活性存在于（NH 4）2SO 4沉淀的细胞上清液和沉淀与六臂体在甘油梯度。然而，通过抗体吸附纯化的Hexabrachions已经失去了这种活性，这表明这是由于一个单独的因素与hexabrachions在梯度馏分。几项先前无关的研究的综合信息表明，六臂体可能在组织细胞外基质的局部区域中发挥作用。该蛋白在胚胎发育期间的特定时间和位置显著表达，保留在某些成人组织中，并在多种肿瘤中重新表达。
Cell cultures of chicken embryo and human fibroblasts produce a large extracellular matrix molecule with a six-armed structure that we called a hexabrachion (Erickson, H. P., and J. L. Iglesias, 1984, Nature (Lond.), 311:267-269. In the present work we have determined that the myotendinous (M1) antigen described by M. Chiquet and D. M. Fambrough in chicken tissues (1984, J. Cell Biol., 98:1926-1936), and the glioma mesenchymal extracellular matrix protein described by Bourdon et al. in human tumors (Bourdon, M. A., C. J. Wikstrand, H. Furthmayr, T. J. Matthews, and D. D. Bigner, 1983, Cancer Res. 43:2796-2805) have the structure of hexabrachions. We also demonstrate that the M1 antigen is present in embryonic brain, where it was previously reported absent, and have purified hexabrachions from brain homogenates. The recently described cytotactin (Grumet, M., S. Hoffman, K. L. Crossin, and G. M. Edelman, 1985, Proc. Natl. Acad. Sci. USA, 82:8075-8079) now appears to be identical to the chicken hexabrachion protein. In a search for functional roles, we looked for a possible cell attachment activity. A strong, fibronectin-like attachment activity was present in (NH4)2SO4 precipitates of cell supernatant and sedimented with hexabrachions in glycerol gradients. Hexabrachions purified by antibody adsorption, however, had lost this activity, suggesting that it was due to a separate factor associated with hexabrachions in the gradient fractions. The combined information in the several, previously unrelated studies suggests that hexabrachions may play a role in organizing localized regions of extracellular matrix. The protein is prominently expressed at specific times and locations during embryonic development, is retained in certain adult tissues, and is reexpressed in a variety of tumors.