Supercritical carbon dioxide extraction of ubiquinones and menaquinones from activated sludge
Supercritical carbon dioxide extraction of ubiquinones and menaquinones from activated sludge
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DOI：
10.1016/j.chroma.2006.01.112
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发表时间：
2006-04-28
影响因子：
4.1
通讯作者：
Fujie, K
中科院分区：
文献类型：
--
作者：
Irvan;Atsuta, Y;Fujie, K
采用超临界CO2 (scCO(2))萃取法，以甲醇为修饰剂，测定活性污泥中泛醌和甲基萘醌的含量。根据 0.1 g 干燥活性污泥中的保留时间和 UV 光谱，鉴定出 4 种泛醌和 12 种甲基萘醌。最佳提取条件为压力25 MPa、温度55 ℃、10%（v/v）甲醇15 min。在此条件下，提取的泛醌和甲基萘醌的浓度分别为0.181和0.326μmol/g-干细胞。结果与基于多样性和相异指数的有机溶剂提取获得的结果相当。此外，该方法还根据重复性进行了评估，其 RSD 为
Supercritical CO2 (scCO(2)) extraction, with methanol as modifier, was applied to the determination of ubiquinones and menaquinones in activated sludge. Four ubiquinones and 12 menaquinones species were identified based on retention time and UV spectrum in 0.1 g dried activated sludge. The optimum extraction conditions were at a pressure of 25 MPa, a temperature of 55 degrees C, and 10% (v/v) methanol for 15 min. At this condition, the concentrations of extracted ubiquinones and menaquinones were found to be 0.181 and 0.326 mu mol/g-dry-cell, respectively. The results were comparable with those obtained by organic solvent extraction based on diversity and dissimilarity indices. Furthermore, the method was evaluated in term of repeatability, which resulted in an RSD of