A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila
A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila
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DOI：
10.1016/s0092-8674(00)81676-1
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发表时间：
1999-12-23
期刊：
影响因子：
64.5
通讯作者：
Taghert, PH
中科院分区：
文献类型：
--
作者：
Renn, SCP;Park, JH;Taghert, PH
昼夜节律起搏器系统传输定时信息以控制行为的机制在很大程度上是未知的。在这里，我们定义了果蝇该机制的两个关键特征。我们首先描述了 pdf 神经肽基因的动物突变体，该基因由大多数候选起搏器（LN，神经元）表达。接下来，我们描述了 pdf 神经元被选择性消融的动物。两组动物都产生了相似的行为表型。两组都受到光的影响，但在恒定条件下两者基本上都没有节律。每个 pdf 变体中都有少数表现出弱到中度的自由运行节律性。这些结果证实了 LN 神经元作为控制果蝇日常运动的主要昼夜节律起搏器。他们还暗示 PDF 是主要的昼夜节律发射器。
The mechanisms by which circadian pacemaker systems transmit timing information to control behavior are largely unknown. Here, we define two critical features of that mechanism in Drosophila. We first describe animals mutant for the pdf neuropeptide gene, which is expressed by most of the candidate pacemakers (LN, neurons). Next, we describe animals in which pdf neurons were selectively ablated. Both sets of animals produced similar behavioral phenotypes. Both sets entrained to light, but both were largely arrhythmic under constant conditions. A minority of each pdf variant exhibited weak to moderate free-running rhythmicity. These results confirm the assignment of LN, neurons as the principal circadian pacemakers controlling daily locomotion in Drosophila. They also implicate PDF as the principal circadian transmitter.