IMMUNOLOGICAL COMPARISON OF THE USUAL AND ATYPICAL HUMAN-SERUM CHOLINESTERASE PHENOTYPES
IMMUNOLOGICAL COMPARISON OF THE USUAL AND ATYPICAL HUMAN-SERUM CHOLINESTERASE PHENOTYPES
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DOI：
10.1007/bf00498901
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发表时间：
1983-01-01
影响因子：
2.4
通讯作者：
LADU, BN
中科院分区：
文献类型：
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作者：
ECKERSON, HW;OSEROFF, A;LADU, BN
针对高度纯化的普通人血清胆碱酯酶（最常见的表型）制备的抗血清与非典型血清胆碱酯酶交叉反应相同。循环的非典型酶蛋白的水平，免疫学测定，是约30%的降低时，酶来自一个非典型的，而不是一个常见的表型，和酶的活性水平测定酶在Vmax与邻硝基苯丁酸酯或苯甲酰胆碱作为底物表现出大致相同程度的减少。来自28个正常个体和20个非典型个体的血清中的平均比活性（Vmax/μ g酶蛋白的活性）没有显著差异。非典型酶不仅具有改变的催化特性（Km），而且可能比普通酶合成得更慢，或在体内清除得更快。
Antiserum prepared against highly purified usual human serum cholinesterase (the most common phenotype) cross-reacted identically with the atypical serum cholinesterase. The level of circulating atypical enzyme protein, determined immunologically, was about 30% lower when the enzyme came from an atypical rather than a usual phenotype, and the level of enzyme activity measured enzymatically at Vmax with either o-nitrophenylbutyrate or benzoylcholine as substrate showed approximately the same degree of reduction. The average specific activity (activity at Vmax/.mu.g of enzyme protein) in sera from 28 usual and 20 atypical individuals did not differ significantly. The atypical enzyme not only has altered catalytic properties (Km) but also might be synthesized more slowly, or cleared in vivo more rapidly, than the usual enzyme.