Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry
Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry
复制标题
DOI：
10.1016/j.chroma.2011.09.043
复制
发表时间：
2011-11-11
影响因子：
4.1
通讯作者：
Bai, Fali
中科院分区：
文献类型：
--
作者：
Guan, Wenna;Zhao, Hui;Bai, Fali
脂肪酸基生物燃料的简单、快速定量测定对微藻生产生物燃料的基因工程进展研究具有重要意义。从生物系统中生产的理想生物燃料在化学上应该与石油相似，比如脂肪酸分子，包括游离脂肪酸、脂肪酸甲酯、脂肪酸乙酯、脂肪醇和脂肪烷烃。本研究建立了一种气相色谱-质谱（GC-MS）同时定量野生型聚囊菌PCC 6803及其基因工程菌株产生的7种游离脂肪酸、9种脂肪酸甲酯、5种脂肪酸乙酯、5种脂肪醇和3种脂肪烷烃的方法。采用内标峰面积比较对GC-MS分析获得的数据进行量化。对该方法的线性度、检出限（LOD）和精密度（RSD）进行了评价。结果表明，脂肪酸基生物燃料可直接通过气相色谱-质谱法测定，无需衍生。因此，使用本工作建立的GC-MS方法可以实现对野生型和基因工程蓝藻生产的脂肪酸基生物燃料的快速可靠的定量分析。(C) 2011 Elsevier B.V.版权所有
Simple and rapid quantitative determination of fatty-acid-based biofuels is greatly important for the study of genetic engineering progress for biofuels production by microalgae. Ideal biofuels produced from biological systems should be chemically similar to petroleum, like fatty-acid-based molecules including free fatty acids, fatty acid methyl esters, fatty acid ethyl esters, fatty alcohols and fatty alkanes. This study founded a gas chromatography-mass spectrometry (GC-MS) method for simultaneous quantification of seven free fatty acids, nine fatty acid methyl esters, five fatty acid ethyl esters, five fatty alcohols and three fatty alkanes produced by wild-type Synechocystis PCC 6803 and its genetically engineered strain. Data obtained from GC-MS analyses were quantified using internal standard peak area comparisons. The linearity, limit of detection (LOD) and precision (RSD) of the method were evaluated. The results demonstrated that fatty-acid-based biofuels can be directly determined by GC-MS without derivation. Therefore, rapid and reliable quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria can be achieved using the GC-MS method founded in this work. (C) 2011 Elsevier B.V. All rights reserved.