Nucleic acid hybridization for the detection of viral genomes.
Nucleic acid hybridization for the detection of viral genomes.
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用于检测病毒基因组的核酸杂交。
DOI：
10.1007/978-3-642-68949-9_18
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发表时间：
1983
影响因子：
--
通讯作者：
L. Gissmann
中科院分区：
文献类型：
--
作者：
G. Bornkamm;C. Des̀granges;L. Gissmann
单链核酸在适当条件下遇到互补序列，可以形成稳定的双链。这种反应被称为杂交，在过去的15年中被广泛用于探索细胞和病毒基因的结构和表达。特别是随着分子克隆技术的进步，特异探针的杂交技术使得从基因组文库中分离和研究单个基因成为可能，从而对我们对真核生物基因组织的理解做出了重大贡献。
Single-stranded nucleic acids can form stable duplexes if they encounter complementary sequences under appropriate conditions. This reaction is called hybridization and has been extensively used during the past 15 years to explore the structure and expression of cellular and viral genes. Particularly with the advance of molecular cloning, hybridization with specific probes has made it possible to isolate and study individual genes from genomic libraries and has thus contributed significantly to our understanding of eukaryotic gene organization.