Ethanol addition on inactivation of Saccharomyces pastorianus by a two‐stage system with low‐pressure carbon dioxide microbubbles can accelerate the cell membrane injury
Ethanol addition on inactivation of Saccharomyces pastorianus by a two‐stage system with low‐pressure carbon dioxide microbubbles can accelerate the cell membrane injury
复制标题
添加乙醇低压二氧化碳微泡两级系统灭活巴斯德酵母可加速细胞膜损伤
DOI：
10.1002/btpr.2579
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发表时间：
2018
影响因子：
2.9
通讯作者：
S. Odake
中科院分区：
文献类型：
--
作者：
F. Kobayashi;S. Odake
研究了乙醇对采用低压二氧化碳微泡（两级MBCO 2）的两级系统灭活巴斯德酵母的影响。S的对数减少值为0和>5。分别悬浮在含有0%和10%乙醇的生理盐水（PS）中的巴斯德菌群通过两阶段MBCO 2在1 MPa的混合容器压力和40°C的加热盘管温度下发生。相反，检测到的存活S.酵母菌和霉菌琼脂（YMA，最适琼脂）在含5%乙醇的PS培养基中对巴斯德菌细胞的生长抑制作用高于含2.5%氯化钠的YMA，其次是酵母氮基琼脂（YNBA，最低琼脂）。用荧光偏振法研究了S.在含有5%和10%乙醇的PS中的巴斯德菌的生长与在两阶段MBCO 2的加热线圈中的暴露时间相似地增加，并且与在YNBA中测量的存活细胞数相关。S.在含有5%乙醇的PS中的巴斯德菌的生长速率随着在两阶段MBCO 2的加热盘管中的暴露时间线性降低。此外，S.在含有10%乙醇的PS中，在加热盘管中暴露1 min时，两阶段MBCO 2显著引起巴斯德菌的灭活，但随后保持恒定直至5 min，与灭活效率一致。因此，S. pastorianus悬浮液建议加速由两阶段MBCO 2引起的细胞膜损伤。© 2017美国化学工程师学会生物技术。程序，34：282-286，2018
The effect of ethanol on the inactivation of Saccharomyces pastorianus by a two‐stage system with low‐pressure carbon dioxide microbubbles (two‐stage MBCO2) was investigated. Zero and >5 log reductions of S. pastorianus populations suspended in physiological saline (PS) containing 0% and 10% ethanol, respectively, occurred by the two‐stage MBCO2 at a mixing vessel pressure of 1 MPa and a heating coil temperature of 40°C. Conversely, the detected number of surviving S. pastorianus cells in PS containing 5% ethanol was higher in yeast and mold agar (YMA, an optimum agar) than YMA with 2.5% sodium chloride, followed by yeast nitrogen base agar (YNBA, a minimum agar). The fluorescence polarization of S. pastorianus in PS containing 5% and 10% ethanol increased similarly with exposure time in the heating coil of two‐stage MBCO2 and was correlated with the surviving cell number measured in YNBA. The intracellular pH (pHin) of S. pastorianus in PS containing 5% ethanol decreased linearly with exposure time in the heating coil of two‐stage MBCO2. Also, the pHin‐lowering of S. pastorianus in PS containing 10% ethanol was drastically caused by two‐stage MBCO2 at 1 min exposure time in the heating coil but then stayed constant until 5 min, agreeing with the inactivation efficiency. Therefore, ethanol in S. pastorianus suspension was suggested to accelerate the cell membrane injury caused by two‐stage MBCO2. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:282–286, 2018