Three-Dimensional Immobilization of Bacterial Cells with a Fibrous Network and Its Application in a High-Rate Fixed-Bed Nitrifying Bioreactor
Three-Dimensional Immobilization of Bacterial Cells with a Fibrous Network and Its Application in a High-Rate Fixed-Bed Nitrifying Bioreactor
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纤维网络三维固定化细菌细胞及其在高速固定床硝化生物反应器中的应用
DOI：
10.1252/jcej.35.68
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发表时间：
2002
影响因子：
0.8
通讯作者：
A. Hirata
中科院分区：
文献类型：
--
作者：
H. Hayashi;M. Ono;S. Tsuneda;A. Hirata
提出了一种利用纤维材料固定化硝化细菌的新技术——纤维网络三维固定化（3-D IFN），并对其在高速硝化生物反应器中的应用进行了研究。所采用的纤维载体是镍铁纤维渣（FS），它是镍铁电冶炼过程产生的工业固体废物。由于用FS固定细胞可以在90秒内完成，因此这种方法比任何其他细胞固定技术都要快得多。采用间歇式和连续进料两种试验方法对固定化FS填充的固定床硝化反应器进行了研究。通过控制循环流速，改善了溶解氧向固定化细胞的输送，这是该反应器的一个显著特点。连续进料试验表明，该反应器的氨去除率达到6.5 kg-N/（m3-反应器）/d，与常规固定床反应器相比，氨去除率极高。结果表明，三维IFN是一种简单有效的细胞固定化技术，可成功应用于高速率硝化反应器。
A novel technique of immobilizing nitrifying bacteria using fibrous material, the three-dimensional immobilization with a fibrous network (3-D IFN), is proposed and its use in a high-rate nitrifying bioreactor is investigated. The fibrous carrier employed is ferro-nickel fibrous slag (FS), which is industrial solid waste from a ferro-nickel electrosmelting process. Since cell immobilization with FS is readily carried out within 90 seconds, this method is by far more rapid than any other cell immobilization techniques. A fixed-bed nitrifying reactor packed with cell-immobilizing FS is examined by both batch-mode and continuous feeding tests. By controlling the circulation flow rate, the transport of dissolved oxygen to the immobilized cells is improved, which is a distinct characteristic of this reactor. The continuous feeding test revealed that the ammonia removal rate of the reactor reached 6.5 kg-N/(m3-reactor)/d, which was extremely high compared with that of the conventional fixed-bed reactor for wastewater treatment. It is demonstrated that the 3-D IFN is a simple yet effective cell immobilization technique that can be successfully used in a high-rate nitrifying reactor.