Stimulation by phospholipid of calcium-dependent phosphorylation of endogenous proteins from mammalian tissues.
Stimulation by phospholipid of calcium-dependent phosphorylation of endogenous proteins from mammalian tissues.
复制标题
磷脂刺激哺乳动物组织内源蛋白的钙依赖性磷酸化。
DOI：
10.1016/0304-4165(81)90195-1
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Kuo,JF
中科院分区：
文献类型：
--
作者：
Wrenn,RW;Katoh,N;Kuo,JF
在几种组织的提取液中，检测了由磷脂或钙调蛋白增强的钙依赖蛋白激酶的内源性底物蛋白的存在。胰腺、输精管、肾上腺和肝脏中含有磷脂敏感蛋白激酶底物蛋白。胰腺底物蛋白对磷脂敏感蛋白激酶的磷酸化迅速且对钙离子高度敏感，在暴露于钙离子和磷脂酰丝氨酸后15 S内即可检测到，并可在低至0.5μM的钙离子浓度下检测到。这些发现表明，磷脂敏感的蛋白激酶系统可能在多种哺乳动物细胞中介导钙离子的某些作用。
The occurrence of endogenous substrate proteins for calcium-dependent protein kinase, augmented by either phospholipid or calmodulin, was examined in extracts of several tissues. Pancreas, vas deferens, adrenal and liver were found to contain substrate proteins for phospholipid-sensitive protein kinase. Phosphorylation of pancreatic substrate protein for phospholipid-senstivie protein kinase was rapid and highly sensitive to Ca2+, being detectable within 15 s a following exposure to Ca2+and phosphatidylserine and at concentrations of Ca2+as low as 0.5 μM. These findings suggest that the phospholipid-sensitive protien kinase system may serve to mediate some effects of Ca2+in a variety of mammalian cell types.