Target cell specificity of a bacteriocin molecule: A C-terminal signal directs lysostaphin to the cell wall of Staphylococcus aureus
Target cell specificity of a bacteriocin molecule: A C-terminal signal directs lysostaphin to the cell wall of Staphylococcus aureus
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DOI：
10.1002/j.1460-2075.1996.tb00859.x
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发表时间：
1996-09-16
期刊：
影响因子：
11.4
通讯作者：
Schneewind, O
中科院分区：
文献类型：
--
作者：
Baba, T;Schneewind, O
微生物分泌抗生素，导致特定靶细胞的选择性破坏。尽管许多抗生素的作用方式是已知的，但迄今为止尚未描述这些分子特异性针对其靶细胞的机制，但模拟葡萄球菌分泌溶葡萄球菌素，这是一种溶菌酶，通常裂解葡萄球菌肽聚糖，但专门针对金黄色葡萄球菌靶细胞。足以使细菌素以及杂合指示蛋白与金黄色葡萄球菌细胞壁结合的序列元件由 92 个 C 端溶葡萄球菌素残基组成。当杂合指示分子从外部添加到细菌时或当它们被合成并通过 N 端前导肽从其细胞质输出时，就会发生对金黄色葡萄球菌细胞壁的靶向。缺乏 C 端靶向信号的溶葡萄球菌素分子具有酶活性，但失去了区分金黄色葡萄球菌和模拟葡萄球菌细胞的能力，表明该结构域的功能是赋予溶葡萄球菌分子靶细胞特异性。
Microbial organisms secrete antibiotics that cause the selective destruction of specific target cells. Although the mode of action is known for many antibiotics, the mechanisms by which these molecules are directed specifically to their target cells hitherto have not been described, Staphylococcus simulans secretes lysostaphin, a bacteriolytic enzyme that cleaves staphylococcal peptidoglycans in general but that is directed specifically to Staphylococcus aureus target cells. The sequence element sufficient for the binding of the bacteriocin as well as of hybrid indicator proteins to the cell wall of S.aureus consisted of 92 C-terminal lysostaphin residues, Targeting to the cell wall of S.aureus occurred either when the hybrid indicator molecules were added externally to the bacteria or when they were synthesized and exported from their cytoplasm by an N-terminal leader peptide. A lysostaphin molecule lacking the C-terminal targeting signal was enzymatically active but had lost its ability to distinguish between S.aureus and S.simulans cells, indicating that this domain functions to confer target cell specificity to the bacteriolytic molecule.