Connective tissue changes in ileal Crohn's disease: Relationship to disease phenotype and ulcer-associated cell lineage
Connective tissue changes in ileal Crohn's disease: Relationship to disease phenotype and ulcer-associated cell lineage
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DOI：
10.1007/bf02234738
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发表时间：
2001-03-01
影响因子：
3.9
通讯作者：
Warren, BF
中科院分区：
文献类型：
--
作者：
Borley, NR;Mortensen, NJM;Warren, BF
目的：克罗恩病中肠胶原和平滑肌细胞含量的异常已被记录。我们使用免疫组织化学和图像分析研究了结缔组织变化、疾病“类型”和其他疾病特征之间的关系。方法：使用常规组织病理学检查对二十个连续的克罗恩病回肠切除标本和十个正常回肠末端标本进行评估。使用针对平滑肌肌动蛋白和 III 型胶原纤维的单克隆抗体，通过图像分析来确定这些成分占据的粘膜下层面积的百分比。结果：狭窄、穿孔和溃疡样本之间的平滑肌含量没有显着差异。与其他类型相比，狭窄型患者的粘膜下 III 型胶原蛋白含量显着增加。与平滑肌细胞含量相关的唯一因素是存在的溃疡相关细胞谱系的数量。结论：III 型胶原纤维沉积增加而非平滑肌增殖与狭窄表型相关。 III 型胶原纤维的损失可能在穿孔并发症的发生中发挥作用。我们没有发现任何证据表明平滑肌细胞是 III 型胶原纤维产生的来源，尽管有证据表明溃疡相关细胞谱系可能与导致粘膜下新生肌肉化的刺激有关。
PURPOSE: Abnormalities of enteric collagen and smooth-muscle cell content hare been documented in Crohn's disease. We studied the relationships among connective tissue changes, disease "type," and other disease features using immunohistochemistry and image analysis. METHODS: Twenty consecutive ileal resections for Crohn's disease and ten normal terminal ileal specimens were evaluated using conventional histopathologic examination. Monoclonal antibodies to smooth-muscle actin and Type III collagen fibers were used to determine the percentage area of the submucosa occupied by these constituents using image analysis. RESULTS: There were no significant differences in smooth-muscle content among stenosed, perforated, and ulcerated specimens. There was a significantly increased submucosal Type III collagen content in stenosed vs, other types. The only factor that correlated with smooth muscle cell content was the amount of ulcer-associated cell lineage present. CONCLUSIONS: Increased deposition of Type III collagen fibers rather than smooth-muscle proliferation is associated with a stenotic phenotype. Loss of Type III collagen fibers may play a role in the development of perforating complications. We hale found no evidence that smooth-muscle cells are the source of Type III collagen fiber production although there is evidence that ulcer-associated cell lineage may be related to the stimulus leading to submucosal neomuscularization.