MAINTENANCE OF AMELOGENIN GENE-EXPRESSION BY TRANSFORMED EPITHELIAL-CELLS OF MOUSE ENAMEL ORGAN
MAINTENANCE OF AMELOGENIN GENE-EXPRESSION BY TRANSFORMED EPITHELIAL-CELLS OF MOUSE ENAMEL ORGAN
复制标题
DOI：
10.1016/0003-9969(92)90110-t
复制
发表时间：
1992-10-01
影响因子：
3
通讯作者：
SNEAD, ML
中科院分区：
文献类型：
--
作者：
CHEN, LS;COUWENHOVEN, RI;SNEAD, ML
采用电穿孔法将外源基因导入小鼠釉器官上皮细胞。确定了电穿孔的最佳条件。 将携带来自多瘤病毒的大T抗原的编码区的质粒构建体引入EOE细胞中允许建立具有以下特征的衍生细胞系：（1）细胞可以传代多次;（2）它们表达含有角蛋白的细胞骨架;和（3）约100 μ g/ml的细胞。60%的细胞表达釉原蛋白，一种成釉细胞特有的组织特异性基因产物。这种细胞系的潜在用途包括分析：（1）时间和空间限制性表达釉原蛋白所需的上游调控区;（2）同步化细胞中釉原蛋白的翻译后修饰和（3）单层培养中釉质细胞外基质的组织和生物矿化。
Electroporation was used to introduce foreign genes into cells derived from the mouse enamel organ epithelia (EOE). Optima conditions for this electroporation were established. The introduction of a plasmid construct bearing the coding region for the large T-antigen from polyoma virus into EOE cells permitted the establishment of a derivative cell line that has the following characteristics: (1) the cells could be passaged many times; (2) they expressed a keratin-containing cytoskeleton; and (3) approx. 60% of the cells expressed amelogenin, a tissue-specific gene product unique to ameloblasts. Potential uses for such a cell line include analysis of: (1) the upstream regulatory regions required for temporally and spatially restricted expression of amelogenin; (2) the post-translational modification of amelogenin in synchronized cells and (3) the organization and biomineralization of enamel extracellular matrix in monolayer culture.