Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension.
Fibroblast growth factor promotes survival of dissociated hippocampal neurons and enhances neurite extension.
复制标题
成纤维细胞生长因子促进分离的海马神经元的存活并增强神经突的延伸。
DOI：
10.1073/pnas.83.9.3012
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发表时间：
1986
影响因子：
11.1
通讯作者：
Guillemin,R
中科院分区：
文献类型：
--
作者：
Walicke,P;Cowan,WM;Ueno,N;Baird,A;Guillemin,R
碱性成纤维细胞生长因子（FGF）已被发现，增加神经元的存活和神经突起的延伸在一个高度纯化的人口胎鼠海马神经元在明确定义的无血清细胞培养条件下。在FGF的存在下，在简单的塑料基质上培养7天后的神经元存活增加4倍，达到初始群体的54%。使用多聚鸟氨酸-层粘连蛋白的存活率增加2倍至40%。当FGF与塑料或肝素底物结合时，神经突生长显著增加至与层粘连蛋白相当的长度;然而，FGF对层粘连蛋白的神经突生长没有进一步增加。在FGF浓度约为15 pg/ml（1 pM）时观察到半数最大存活;在约375 pg/ml（20 pM）时发生半数最大过程生长。通过破伤风毒素标记和神经丝抗体以及神经元特异性烯醇化酶抗体，将反应细胞鉴定为神经元。星形胶质细胞，确定存在的胶质细胞酸性蛋白，构成约10%的细胞在1周的存在和不存在的FGF。这些结果强烈表明，除了已知的对非神经元细胞的促有丝分裂作用，FGF对海马神经元具有神经营养活性。
Basic fibroblast growth factor (FGF) has been found to increase neuronal survival and neurite extension in a highly purified population of fetal rat hippocampal neurons under well-defined serum-free cell culture conditions. In the presence of FGF, neuronal survival after 7 days in culture on a simple plastic substrate is increased 4-fold, to 54% of the initial population. Survival is increased 2-fold to 40% on polyornithine-laminin. When FGF was bound to plastic or heparin substrates, neurite outgrowth was significantly increased to lengths comparable to those seen with laminin; however, FGF produced no further increase in neurite outgrowth on laminin. Half-maximal survival was observed at FGF concentrations of about 15 pg/ml (1 pM); half-maximal process outgrowth occurred at about 375 pg/ml (20 pM). The responsive cells were identified as neurons by their labeling with tetanus toxin and by antibodies to neurofilaments and to the neuron-specific enolase. Astrocytes, identified by the presence of glial fibrillary acidic protein, constituted about 10% of cells present at 1 week both in the presence and in the absence of FGF. These results strongly suggest that, in addition to its known mitogenic effects on nonneuronal cells, FGF possesses neurotrophic activity for hippocampal neurons.