Evidence for the inhibitory role of guanosine 3', 5'-monophosphate in ADP-induced human platelet aggregation in the presence of nitric oxide and related vasodilators.
Evidence for the inhibitory role of guanosine 3', 5'-monophosphate in ADP-induced human platelet aggregation in the presence of nitric oxide and related vasodilators.
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DOI：
10.1182/blood.v57.5.946.bloodjournal575946
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发表时间：
1981-05
期刊：
影响因子：
20.3
通讯作者：
B. T. Mellion;L. J. Ignarro;E. H. Ohlstein;E. Pontecorvo;A. Hyman;P. Kadowitz
中科院分区：
文献类型：
--
作者：
B. T. Mellion;L. J. Ignarro;E. H. Ohlstein;E. Pontecorvo;A. Hyman;P. Kadowitz
先前的观察发现血小板中的鸟苷3′。5 ' -单磷酸（环状GMP）的积累发生在血小板聚集过程中，也发生在硝普苷存在的情况下。抑制聚集。促使对硝普赛的效果进行评估。一氧化氮。及相关药物对人血小板聚集的影响。采用二磷酸腺苷（ADP）诱导富血小板血浆中血小板不可逆聚集。硝普酸。一氧化氮。和N-methyl-N -nitro-N-nitrosoguanidine。以浓度依赖的方式。抑制adp诱导的血小板聚集。显著激活可溶性血小板鸟苷酸环化酶（70-280倍）。血小板循环GMP水平明显升高（100-200倍）。香烟烟雾的气相它含有一氧化氮。还能抑制血小板聚集和提高血小板环GMP水平。除了衰减聚集。这些药物能有效分解血小板。这与伴随而来的
Previous observations that platelet guanosine 3’. 5’-monophosphate (cyclic GMP) accumulation occurred during platelet aggregation and also in the presence of nitroprusside. which inhibits aggregation. prompted an evaluation of the effects of nitroprusside. nitric oxide. and related agents on human platelet aggregation. Adenosine diphosphate (ADP) was employed to induce irreversible aggregation of human platelets in platelet-rich plasma. Nitroprusside. nitric oxide. and N-methyl-N’-nitro-N-nitrosoguanidine. in a concentration-dependent manner. inhibited ADPinduced platelet aggregation. markedly activated soluble platelet guanylate cyclase (70-280-fold). and markedly elevated platelet cyclic GMP levels (100-200-fold). The gaseous phase of cigarette smoke. which contains nitric oxide. also inhibited platelet aggregation and elevated platelet cyclic GMP levels. In addition to attenuating aggregation. these agents were effective in disaggregating platelets. which was associated with a concomitant rise in