Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV-related squamous cell carcinomas of the oropharynx
Correlation of p16 immunohistochemistry in FNA biopsies with corresponding tissue specimens in HPV-related squamous cell carcinomas of the oropharynx
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DOI：
10.1002/cncy.21600
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发表时间：
2015-12-01
影响因子：
3.4
通讯作者：
Bernadt, Cory T.
中科院分区：
文献类型：
--
作者：
Jalaly, Jalal B.;Lewis, James S.;Bernadt, Cory T.
背景：人乳头瘤病毒（HPV）相关的口咽鳞状细胞癌（SCC）是一种独特的癌症，对预后和治疗具有重要意义。免疫组织化学（IHC）检测p16（也称为细胞周期蛋白依赖性激酶抑制剂2A，多重肿瘤抑制因子1）被用作转录活性高风险HPV的替代标志物。本研究的主要目的是将细针穿刺（FNAs）细胞块的p16免疫组化与hpv相关口咽鳞状细胞癌的手术病理标本相关联。方法共鉴定了48例诊断为口咽或非口咽鳞状细胞癌的患者，并且FNA显示转移性鳞状细胞癌具有可用的细胞阻断材料。在FNA细胞块和手术病理标本上评估p16的免疫组化。在31个FNA细胞块上进行高危HPV信使RNA的原位杂交。结果虽然大多数细胞块中都观察到部分p16染色，但当FNA细胞块材料中至少15%的肿瘤细胞存在p16强阳性染色时，48个FNA中有47个（98%）与手术病理标本一致。此外，高危HPV RNA原位杂交与手术病理标本（96%）和FNAs（93%）的p16染色高度相关。结论FNA细胞块的p16免疫组化与手术病理标本有很好的相关性，细胞块中至少有15%的阳性染色。手术病理标本的推荐阈值（70%阳性染色）如果应用于FNA细胞块，可能会产生很高的假阴性结果率。癌症（Cancer Cytopathol）； 2015;123:723-731。(c) 2015美国癌症协会。本文报道了细针穿刺活检细胞块的p16免疫组化与人乳头瘤病毒相关口咽鳞状细胞癌手术病理标本的比较。两种方法之间有很好的相关性，在细胞块中使用至少15%的焦点融合染色。
BACKGROUNDHuman papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (SCC) is a unique form of carcinoma that is important to identify for prognosis and treatment. Immunohistochemistry (IHC) for p16 (also known as cyclin-dependent kinase inhibitor 2A, multiple tumor suppressor 1) is used as a surrogate marker for transcriptionally active, high-risk HPV. The primary objective of this study was to correlate p16 IHC of cell blocks from fine-needle aspirations (FNAs) with surgical pathology specimens of HPV-related oropharyngeal SCC.METHODSIn total, 48 patients who had a diagnosis of oropharyngeal or nonoropharyngeal SCC and also had an FNA that demonstrated metastatic SCC with available cell block material were identified. IHC for p16 was evaluated on both FNA cell blocks and surgical pathology specimens. In situ hybridization for high-risk HPV messenger RNA was performed on 31 of the FNA cell blocks.RESULTSAlthough partial p16 staining was observed in the majority of cell blocks, there was concordance in 47 of 48 FNAs (98%) with surgical pathology specimens when strong positive p16 staining of at least 15% of tumor cells in FNA cell block material was present. In addition, high-risk HPV RNA in situ hybridization demonstrated a high correlation with p16 staining in surgical pathology specimens (96%) and FNAs (93%).CONCLUSIONSThere was excellent correlation between p16 IHC of FNA cell blocks and surgical pathology specimens using a cutoff of at least 15% positive staining in cell blocks. The recommended threshold (70% positive staining) for surgical pathology specimens may yield a high rate of false-negative results if applied to FNA cell blocks. Cancer (Cancer Cytopathol) 2015;123:723-731. (c) 2015 American Cancer Society.An evaluation of p16 immunohistochemistry of cell blocks from fine-needle aspiration biopsies compared with surgical pathology specimens of human papillomavirus-related oropharyngeal squamous cell carcinoma is reported. There is excellent correlation between the 2 methods using a cutoff of at least 15% focal confluent staining in cell blocks.