Human platelet lysate contains growth factor activities for established cell lines derived from various tissues of several species.
Human platelet lysate contains growth factor activities for established cell lines derived from various tissues of several species.
复制标题
人血小板裂解物含有针对源自多个物种的各种组织的已建立细胞系的生长因子活性。
DOI：
10.1007/bf02619199
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Sirbasku,DA
中科院分区：
文献类型：
--
作者：
Eastment,CT;Sirbasku,DA
已经研究了来自人血小板的因子，这些因子促进对血小板反应的大鼠乳腺癌细胞系MTW 9/PL、BALB/c 3 T3小鼠胚胎成纤维细胞和许多其他已建立的细胞系的生长。检测了各种常用细胞系（包括人、小鼠、猴、鸡、大鼠、中国仓鼠和叙利亚仓鼠来源的细胞系）对标准浓度200 μg/ml人血小板裂解物的生长反应，发现裂解物对29种不同细胞系中的24种具有促有丝分裂活性。比较了MTW 9/PL细胞的血小板生长活性和BALB/c 3 T3细胞的血小板有丝分裂原血小板衍生生长因子（PDGF）。当用高纯度胰蛋白酶消化血小板裂解物时，MTW 9/PL细胞的促有丝分裂活性不受影响，而BALB/c 3 T3细胞的促有丝分裂活性基本上被破坏。通过在100°C下加热人血小板裂解物2分钟，然后澄清、透析、冻干和重构来制备粗PDGF。该PDGF材料对MTW 9/PL细胞没有明显的生长活性，尽管该材料在Biogel P-100上的层析显示出对BALB/c 3 T3细胞的高分子量（约40，000道尔顿）活性（推测为PDGF）和对MTW 9/PL细胞的两种生长活性，一种高分子量活性和分子量小于10，000的第二种活性。这些研究证明了一种形式的上皮肿瘤细胞生长活性，其可与粗加热提取物中的3 T3型PDGF分离。
Factors have been studied from human platelets that promote the growth of a hormone-responsive rat mammary adenocarcinoma cell line MTW9/PL, the BALB/c 3T3 mouse embryo fibroblasts, and numerous other established cell lines. A wide variety of the commonly employed cell lines, including lines of human, mouse, monkey, chicken, rat, Chinese hamster, and Syrian hamster origin, were tested for their growth response to a standard concentration of 200 μg/ml human platelet lysate, and the lysate was found to contain mitogenic activity for 24 of the 29 different lines assayed. A comparison was made between the platelet growth activity for the MTW9/PL cells and the well characterized platelet mitogen for the BALB/c 3T3 cells, platelet-derived growth factor (PDGF). When the platelet lysate was subjected to digestion by highly purified trypsin, the mitogenic activity for the MTW9/PL cells was not affected whereas that for the BALB/c 3T3 cells was essentially destroyed. Crude PDGF was prepared by heating the human platelet lysates at 100°C for 2 min followed by clarification, dialysis, lyophilization, and reconstitution. This PDGF material had no apparent growth activity for MTW9/PL cells, although chromatography of this material on Biogel P-100 revealed a high molecular weight (approximately 40,000 daltons) activity for the BALB/c 3T3 cells (presumably PDGF) and two growth activities for the MTW9/PL cells, one high molecular weight activity and a second activity of molecular weight less than 10,000. These studies demonstrated a form of epithelial tumor cell growth activity separable from the 3T3 type PDGF in crude heated extracts.