Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
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DOI：
10.1292/jvms.10-0263
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Inokuma, Hisashi
中科院分区：
文献类型：
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作者：
Yokoyama, Naoaki;Ueno, Akio;Inokuma, Hisashi
东方泰勒虫是一种良性原生动物，在日本分布广泛，但有时会给养殖业造成严重的经济损失。在这项研究中，我们根据在日本南部地区放牧的牛身上检测到的东方毛滴虫主要胞浆表面蛋白(MPSP)和p23的编码基因进行了分子调查，包括熊本县(麻生县和久间县)的2个农场和冲绳县(石垣岛、伊里奥莫特和横河群岛)的3个农场。诊断用MPSP和p23-PCR检测结果表明，东方毛滴虫在所有牛群中的感染率都很高。系统发育分析发现4个msp型和3个p23型。更重要的是。本研究建立了MPSP基因特异的聚合酶链式反应方法，观察到东方锥虫5个地区的基因型在受检养殖场的广泛分布。结果表明，日本熊本县和冲绳县至少存在5种类型的东方毛滴虫，该方法可用于日本运输牛的检疫和牛泰勒虫病的流行病学调查。
Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore. MPSP genotype-specific PCR methods were developed in this study and wide distributions of 5-district genotypes of T. orientalis were observed for the examined farms. Our results indicate that at least 5 types of T. orientalis exist in Kumamoto and Okinawa prefectures of Japan and that genotype-specific PCR assays are highly applicable for the quarantine of transported cattle and for epidemiological surveys of bovine theileriosis in Japan.