The Haspin gene:: location in an intron of the Integrin αE gene, associated transcription of an Integrin αE-derived RNA and expression in diploid as well as haploid cells
The Haspin gene:: location in an intron of the Integrin αE gene, associated transcription of an Integrin αE-derived RNA and expression in diploid as well as haploid cells
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DOI：
10.1016/s0378-1119(01)00387-0
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发表时间：
2001-04-04
期刊：
影响因子：
3.5
通讯作者：
Higgins, JMG
中科院分区：
文献类型：
--
作者：
Higgins, JMG
Haspin是最近在小鼠中鉴定的丝氨酸/苏氨酸激酶，被认为调节单倍体生殖细胞的细胞周期和分化。在这里，在整合素alphaE基因的内含子内鉴定出haspin基因。转录从双向CpG岛相关启动子发生，该启动子也产生可变剪接的整联蛋白α E衍生的RNA。值得注意的是，人类和小鼠haspin基因缺乏内含子，并具有逆转录酶的特征。人haspin cDNA显示，人和鼠蛋白在C-末端激酶结构域中有83%相同，但在N-末端区域中只有53%相同。haspin激酶结构域的结构特征，区分它从以前的特征蛋白质，并建议haspin是一个新的蛋白激酶家族的成员。尽管以前认为haspin在睾丸中选择性表达，但在胸腺、骨髓、胎肝和其他胎儿组织以及所有检测的增殖细胞系中也以较低水平转录。因此，haspin可能在调节多种组织中的二倍体和单倍体细胞分化中发挥重要作用。(C)2001 Elsevier Science B.V.保留所有权利。
Haspin is a serine/threonine kinase, recently identified in mice, that is thought to regulate cell cycle and differentiation of haploid germ cells. Here, the haspin gene is identified within an intron of the integrin alphaE gene. Transcription occurs from a bi-directional CpG island-associated promoter that also generates an alternatively spliced integrin alphaE derived RNA. Remarkably, the human and murine haspin genes lack introns, and have features of retroposons. The human haspin cDNA reveals that the human and murine proteins are 83% identical in the C-terminal kinase domain, but only 53% identical in the N-terminal region. The haspin kinase domain has structural features that distinguish it from previously characterized proteins and suggest that haspin is a member of a new family of protein kinases. Although formerly thought to be expressed selectively in the testes, haspin is also transcribed at lower levels in thymus, bone marrow, fetal liver and other fetal tissues, and in all proliferating cell lines tested. Thus haspin is likely to be important in regulation of diploid as well as haploid cell differentiation in a variety of tissues. (C) 2001 Elsevier Science B.V. All rights reserved.