SAME OBSERVATIONS ON THE NATURE OF HEATED MIXTURES OF BOVINE SERUM-ALBUMIN WITH AN ALGINATE AND A PECTIN
SAME OBSERVATIONS ON THE NATURE OF HEATED MIXTURES OF BOVINE SERUM-ALBUMIN WITH AN ALGINATE AND A PECTIN
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DOI：
10.1016/0144-8617(94)90035-3
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发表时间：
1994-01-01
影响因子：
11.2
通讯作者：
HARDING, SE
中科院分区：
文献类型：
--
作者：
KELLY, R;GUDO, ES;HARDING, SE
分析超重力中的沉降分析技术，采用相对粘度和动态光散射研究了热处理对牛血清白蛋白（BSA）和海藻酸钠（M（w）约为210 000）以及BSA和高度酯化（70%）果胶（M（w）约为130 000）的稀溶液混合物的影响两种多糖在BSA存在下和单独存在下对热处理显示出非常不同的响应。果胶的动态光散射的等效流体动力学直径在蛋白质的存在下降低，并且混合物和单独的果胶都显示出表观直径随着加热温度的升高而降低。相比之下，藻酸盐混合物在高加热温度下显示出比单独的多糖更大的表观直径，该值似乎对热处理的温度高度敏感。在最高加热温度（95 ° C）下发现最大复合物尺寸，并且高于可以简单地在高温BSA聚集现象方面解释的尺寸。相比之下，在85 ℃下，在与适当的对照比较后，可以通过简单地加权平均游离藻酸盐和聚集的BSA来解释尺寸。
The techniques of sedimentation analysis in the analytical ultracentrifuge, relative viscosity and dynamic light scattering were used to study the effect of heat treatment on dilute solution mixtures of (i) bovine serum albumin (BSA) and a sodium alginate (M(w) similar to 210 000) and (ii) BSA and a highly esterified (70%) pectin (M(w) similar to 130 000).The two polysaccharides showed very different responses to thermal treatment, both in the presence of BSA and alone. The equivalent hydrodynamic diameter from dynamic light scattering of the pectin is reduced in the presence of the protein and both the mixture and pectin alone showed a reduction in apparent diameter with increasing heating temperature. In contrast the alginate mixture showed a larger apparent diameter than the polysaccharide alone at high heating temperatures, a value which appeared to be highly sensitive to the temperature of heat treatment. The maximum complex size was found at the highest heating temperatures (95 degrees C), and higher than can be accounted for simply in terms of high temperature BSA aggregation phenomena. In contrast, at 85 degrees C the size could be accounted for by simply a weighted average of free alginate and aggregated BSA, after comparison with the appropriate controls.