IMPEDED CEREBELLAR DEVELOPMENT AND REDUCED SERUM THYROXINE LEVELS ASSOCIATED WITH FETAL ALCOHOL-INTOXICATION
IMPEDED CEREBELLAR DEVELOPMENT AND REDUCED SERUM THYROXINE LEVELS ASSOCIATED WITH FETAL ALCOHOL-INTOXICATION
复制标题
DOI：
10.1016/0006-8993(79)90785-6
复制
发表时间：
1979-01-01
期刊：
影响因子：
2.9
通讯作者：
YOUNG, B
中科院分区：
文献类型：
--
作者：
KORNGUTH, SE;RUTLEDGE, JJ;YOUNG, B
在妊娠第3天至第20天，将怀孕的白化大鼠置于含有9%乙醇或等量蔗糖的完全液体饮食（Ensure）中。分别于出生后第11天和第14天处死幼鼠，对其小脑和大脑进行形态计量学、组织学和生化分析。在子宫内接触乙醇的幼鼠体重、大脑和小脑明显小于配对喂养的对照组。当体重归一化到成对喂养的对照组时，暴露于酒精的幼崽的小脑质量减少了10%，脑重量减少了3%。第11天和第14天，与对照组相比，乙醇处理幼犬的小脑天冬氨酸转氨酶（AAT, EC 2.6.1.1）活性降低。乙醇处理组血清T4[甲状腺素]水平也降低。组织学分析显示，在出生后11天和14天，乙醇处理的幼崽的外颗粒细胞（EGC）层显著厚于配对喂养的对照组。第11天，乙醇处理幼崽的小脑鸟氨酸脱羧酶（ODC, EC 4.1.1.17）活性高于对照组。体积减小、AAT活性、血清T4水平和EGC层厚度增加表明乙醇处理幼鼠小脑成熟延迟或受阻。这些数据考虑的观点是，乙醇，其他药物，如美沙酮，和产前应激（营养不良）可能通过降低血清T4水平在产后早期（第5-14天）导致小脑成熟延迟。
Pregnant albino rats were placed on a complete liquid diet (Ensure) containing either 9% ethanol or an isocaloric amount of sucrose between the 3rd and 20th day of gestation. The pups born to these rats were sacrificed either day 11 or day 14 postnatum and morphometrical, histological and biochemical analyses were done on their cerebellums and cerebrums. Pups exposed to ethanol in utero had significantly smaller body weights, cerebrums and cerebellums than pair-fed controls. The cerebellar mass was reduced by 10% and the cerebral weight by 3% in the pups exposed to alcohol when body weights were normalized to those of pair-fed controls. Cerebellar aspartyl aminotransferase (AAT, EC 2.6.1.1) activity was reduced at day 11 and 14 in ethanol treated pups compared with controls. Serum T4 [thyroxine] levels were also reduced in the ethanol treated group. Histological analyses revealed that the external granule cell (EGC) layer of ethanol treated pups was significantly thicker at 11 and 14 days postnatum than that of pair-fed control pups. Cerebellar ornithine decarboxylase (ODC, EC 4.1.1.17) activity was higher at day 11 in the ethanol treated pups than in controls. The reduced mass, AAT activity, T4 serum levels and the increased thickness of the EGC layer indicate a delayed or impeded maturation of cerebellum in ethanol treated pups. These data are considered from the viewpoint that ethanol, other drugs such as methadone, and prenatal stress (malnutrition) may cause delayed cerebellar maturation by reducing serum T4 levels in the early postnatal period (day 5-14).