PREFERENTIAL EXPRESSION OF A DEFINED T-CELL RECEPTOR BETA-CHAIN GENE IN HAPTEN-SPECIFIC CYTOTOXIC T-CELL CLONES
PREFERENTIAL EXPRESSION OF A DEFINED T-CELL RECEPTOR BETA-CHAIN GENE IN HAPTEN-SPECIFIC CYTOTOXIC T-CELL CLONES
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DOI：
10.1038/322376a0
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发表时间：
1986-07-24
期刊：
影响因子：
64.8
通讯作者：
EPPLEN, JT
中科院分区：
文献类型：
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作者：
HOCHGESCHWENDER, U;WELTZIEN, HU;EPPLEN, JT
许多不同的抗原可以通过特异性受体被T细胞识别。T细胞受体的α链和β链都有助于抗原识别部分1。β链可变区（Vβ）基因片段的库仅限于大约20个元件2 - 4，这些元件似乎在不同的T细胞中随机使用2，5，6。β-链水平的多样性可以通过几种方式产生2，3，7-12：生殖系基因片段的多样性;通过重排不同的V、多样性（D）、连接（J）和恒定（C）区元件的组合多样性;通过在不同位点连接基因片段的连接多样性; N-区多样性，即在连接位点插入随机核苷酸;以及体细胞突变13。然而，T细胞受体的主要来源和多样性程度尚不清楚。为了解决这一问题，使用特异性寡核苷酸探针对来自C57 BL/6小鼠的42个H-2Kb限制性、2，4，6-三硝基苯基（TNP）特异性细胞毒性T细胞（Tc）克隆的信使RNA中不同β链基因片段的表达进行表征。我们在这里报道，近一半的Tcclones使用相同的元件进行生产性β链基因重排。因此，在这组Tc克隆中，β链基因片段的使用受到限制，这有利于特定的V β-Dβ-Jβ-Cβ与确定的D β元件的组合。
A multitude of different antigens can be recognized by T cells through specific receptors. Both theα- and β-chains of the T-cell receptor contribute to the antigen recognition portion1. The repertoire of β-chain variable region (Vβ) gene segments is limited to some 20 elements2–4which seem to be used randomly in different T cells2,5,6. Diversity at the β-chain level can be created in several ways2,3,7–12: a multiplicity of germline gene segments; combinatorial diversity by rearranging differentV, diversity (D), joining (J) and constant (C) region elements; junctional diversity by joining gene segments at different sites;N-region diversity, that is, insertion of random nucleotides at junctional sites; and somatic mutation13. However, the major sources and the extent of diversity of the T-cell receptor are unclear. To address this issue, 42 H–2Kb-restricted, 2,4,6-trinitrophenyl (TNP)-specific cytotoxic T-cell (Tc) clones from C57BL/6 mice were characterized with respect to expression of different β-chain gene segments in messenger RNA using specific oligonucleotide probes. We report here that nearly half of the Tcclones use identical elements for productive β-chain gene rearrangement. Thus, there is a restriction in the use of β-chain gene segments in this panel of Tcclones which favours a particularVβ–Dβ–Jβ–Cβcombination with a definedDβelement.