An in-tube aptamer/gold nanoparticles coated capillary solid-phase microextraction for separation of adenosine in serum and urine samples
An in-tube aptamer/gold nanoparticles coated capillary solid-phase microextraction for separation of adenosine in serum and urine samples
复制标题
管内适配体/金纳米粒子包被的毛细管固相微萃取用于分离血清和尿液样品中的腺苷
DOI：
10.1016/j.chroma.2019.460617
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发表时间：
2020-01-25
影响因子：
4.1
通讯作者：
Hu, Xiaogang
中科院分区：
文献类型：
--
作者：
Ma, Yanxia;Hao, Lixian;Hu, Xiaogang
腺苷作为一种内源性核苷，对精神分裂症等疾病的诊断和治疗具有重要意义。但由于存在复杂的基体干扰，直接测定生物样品中痕量或超痕量腺苷存在一定困难。本论文建立了一种基于核酸适体/Au纳米粒子涂层的开放式石英毛细管固相微萃取技术，用于生物样品中腺苷的高亲和性分离富集。因此，在开放管状毛细管的内表面涂覆均匀致密的金纳米粒子层，然后将腺苷适体固定在金纳米粒子上，其容量高达每27厘米毛细管2.44微克。结果，与乱序适体/AuNPs涂覆的毛细管相比，毛细管显示出对腺苷的高选择性，选择性因子为14.4。腺苷的提取量也是其结构类似物和对比物如鸟苷、尿苷、胞苷、胸苷和甲苯甲酸的提取量的2.8-24.8倍。在优化提取条件后，建立了核酸适体/金纳米粒子包被固相微萃取-高效液相色谱法测定腺苷的方法，线性范围为0.002-0.100 μ g/mL，检测限为0.45 ng/mL。随后，该方法被应用于人体血清和尿液样品中痕量腺苷的监测。该方法具有较强的降低基体干扰和提高灵敏度的性能，加标回收率分别为89.9-92.6%和91.1-94.5%。(C)2019 Elsevier B. V.版权所有。
As an endogenous nucleoside, adenosine was significant for the diagnosis and treatment of some diseases, such as schizophrenia. However, due to the complicated matrix interference, it was difficult to monitor trace or ultra-trace adenosine directly in bio-samples. In this contribution, a novel in-tube SPME technique based on aptamer/Au nanoparticles coated open tubular fused-silica capillary was established to separate and enrich adenosine in bio-samples with high affinity. Therefore, a uniform and dense AuNPs layer was coated on the inner surface of the open tubular capillary, and then adenosine aptamer was immobilized on AuNPs with a high capacity of 2.44 mu g per 27-cm capillary. As a result, the capillary shown high selectivity to adenosine with a selectivity factor of 14.4 when compared with the scrambled aptamer/AuNPs coated capillary. Also, the extraction amount of adenosine was 2.8-24.8 times higher than those of its structural analogs and contrast, such as guanosine, uridine, cytidine, thymidine, and toluic acid. After the optimization of extraction conditions, the aptamer/AuNPs coated in-tube SPME-HPLC method was developed for the adenosine assay with the linear range of 0.002-0.100 mu g mL(-1) and the detection limit of 0.45 ng mL(-1). Subsequently, the approach was applied for trace adenosine monitoring in human serum and urine samples. It showed a strong performance of reducing matrix interference and improving sensitivity, and the spiking recoveries of 89.9-92.6% and 91.1-94.5% were achieved respectively. (C) 2019 Elsevier B.V. All rights reserved.