In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model
In vitro and in vivo study of PCL/COLL wound dressing loaded with insulin-chitosan nanoparticles on cutaneous wound healing in rats model
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DOI：
10.1016/j.ijbiomac.2018.05.184
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发表时间：
2018-10-01
影响因子：
8.2
通讯作者：
Goodarzi, Arash
中科院分区：
文献类型：
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作者：
Ehterami, Arian;Salehi, Majid;Goodarzi, Arash
在目前的研究中，胰岛素递送壳聚糖纳米颗粒被涂覆到静电纺丝聚（ε-己内酯）（PCL）/胶原（科尔）上，以产生潜在的伤口护理材料。静电纺丝基质由PCL/科尔（1：1（w/w））溶液制备。采用离子凝胶法制备了负载胰岛素的壳聚糖纳米颗粒，并将其附着在纱线上。对敷料的表面润湿性、微观结构、吸水能力、水蒸气渗透性、机械性能、血液相容性、微生物渗透性和细胞行为进行了研究。使用全层切除伤口模型评估敷料的体内愈合能力。我们的数据显示，14天后，与无菌纱布相比，PCL/科尔/Cs-Ins伤口敷料覆盖的伤口可达到几乎完全伤口闭合，无菌纱布显示伤口尺寸缩小近45%。我们的研究结果表明，制造的支架可以潜在地应用于伤口治疗的临床实践。(C)2018爱思唯尔B. V.保留所有权利。
In the current study, insulin delivering chitosan nanoparticles were coated onto the electrospun poly (epsilon-caprolactone) (PCL)/Collagen (COLL) to produce a potential wound care material. Electrospun matrices were fabricated from PCL/COLL (1:1 (w/w)) solution. The insulin-loaded chitosan nanoparticles were produced by ionic gelation process and then attached onto the yarns. The dressings were investigated regarding their surface wettability, microstructure, the capacity to absorb water, water vapour permeability, mechanical properties, blood compatibility, microbial penetration, and cellular behavior. Full-thickness excisional wound model was used to assess the in vivo healing capacity of the dressings. Our data showed that after 14 days the wounds covered with PCL/COLL/Cs-Ins wound dressing could reach to nearly full wound closure compared with the sterile gauze which exhibited nearly 45% of wound size reduction. Our results suggest that fabricated scaffolds can be potentially applied in clinical practice for wound treatment. (C) 2018 Elsevier B.V. All rights reserved.