Effect of inherent epileptic seizures on brain injury after transient cerebral ischemia in Mongolian gerbils
Effect of inherent epileptic seizures on brain injury after transient cerebral ischemia in Mongolian gerbils
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DOI：
10.1007/s00221-003-1655-6
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Nitsch, C
中科院分区：
文献类型：
--
作者：
Herrmann, M;Stern, M;Nitsch, C
阈下兴奋性毒性刺激如短暂脑缺血或化学诱导的癫痫发作可调节随后短暂缺血引起的脑损伤。根据两次损伤之间的延迟，将产生耐受性或累积损伤。我们感兴趣的是蒙古沙鼠中发生的非化学诱导的固有癫痫发作是否对短暂性全脑缺血的结果有影响，即，它们是否是局部缺血实验中的干扰变量。成年雄性沙鼠自发性癫痫发作的发生与视频控制的癫痫发作监测系统进行了登记。在最后一次全身性癫痫发作后2小时或24小时进行双侧颈总动脉闭塞。存活4天后，在海马和纹状体中评估缺血诱导的神经元损伤和胶质细胞活化的程度。缺血2h和24h的沙土鼠脑组织中，甲酚紫染色的神经细胞丢失率无显著性差异。CA1区内皮型一氧化氮合酶的表达随神经元变性而消失。胶质细胞酸性蛋白作为星形胶质细胞的标志物，其上调的分布和程度在两组之间没有差异。我们的结论是，非化学诱导的固有癫痫发作既不保护沙鼠脑损伤，也不增加暂时性前脑缺血造成的损害程度。因此，先天性癫痫发作不会影响损伤的结果，使沙鼠成为研究短暂性脑缺血的可靠模型。
Subthreshold excitotoxic stimuli such as brief cerebral ischemia or chemically induced seizures modulate brain injury resulting from subsequent transient ischemia. Depending on the delay between the two insults, either tolerance or cumulative damage will develop. We were interested whether non-chemically induced inherent epileptic seizures as they occur in Mongolian gerbils have an effect on the outcome of a transient global ischemia, i.e., whether they are an interfering variable in ischemia experiments. Occurrence of spontaneous seizures in adult male gerbils was registered with a video-controlled seizure monitoring system. Bilateral occlusion of common carotid arteries was carried out 2 h or 24 h after the last generalized seizure. After 4 days survival, the extent of ischemia-induced neuronal damage and glial activation were assessed in the hippocampus and striatum. No significant difference in the ischemia induced nerve cell loss was observed in cresyl violet stained sections between the 2-h or 24-h interval gerbils. Neuronal expression of endothelial nitric oxide synthase in CA1 disappeared with neuronal degeneration. Distribution and degree of upregulation of glial fibrillary acidic protein as marker for astrocytes did not differ between the two groups. We concluded that non-chemically induced inherent epileptic seizures neither protect the gerbil brain from injury nor augment the degree of damage resulting from transient forebrain ischemia. Thus, inherent epileptic seizures do not influence the outcome of the insult, making the gerbil a reliable model for studies on transient brain ischemia.