SIMILARITY BETWEEN A UBIQUITOUS PROMOTER ELEMENT IN AN ANCIENT EUKARYOTE AND MAMMALIAN INITIATOR ELEMENTS
SIMILARITY BETWEEN A UBIQUITOUS PROMOTER ELEMENT IN AN ANCIENT EUKARYOTE AND MAMMALIAN INITIATOR ELEMENTS
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DOI：
10.1073/pnas.91.10.4579
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发表时间：
1994-05-10
影响因子：
11.1
通讯作者：
JOHNSON, PJ
中科院分区：
文献类型：
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作者：
QUON, DVK;DELGADILLO, MG;JOHNSON, PJ
为了确定在古代真核生物中起转录起始作用的调控元件，我们分析了迄今为止研究过的最古老的真核生物之一阴道毛滴虫的蛋白质编码基因的上游区域。对来自该原生生物的7个蛋白质编码基因的表征总是揭示了编码区上游存在高度保守的DNA序列基序。这个13-nt基序被证明包围并包含转录起始的精确位点。未发现位于这些基因转录起始位点上游25- 30nt处的典型TATA盒。在哺乳动物体外转录试验中，所有7个阴道绦虫基因的起始位点区域都具有很强的特异性转录起始。在古代真核生物中发现的这一公认的启动子元件在结构和功能上与在高等真核生物的启动子中发现的启动子元件相似。
To identify regulatory elements that play a role in transcription initiation in ancient eukaryotes, we have analyzed the upstream regions of protein coding genes from Trichomonas vaginalis, one of the most ancient eukaryotes studied to date. Characterization of seven protein-coding genes from this protist invariably revealed the presence of a highly conserved DNA sequence motif immediately upstream of the coding region. This 13-nt motif was shown to surround and contain precise sites for transcription initiation. No typical TATA boxes, positioned at 25-30 nt upstream of the transcription start sites of these genes, were found. The start-site regions from all seven T. vaginalis genes impart strong specific initiation of transcription in a mammalian in vitro transcription assay. This consensus promoter element in an ancient eukaryote is similar, both structurally and functionally, to initiator elements found in promoters of higher eukaryotes.