Extracellular Vesicle Flow Cytometry Analysis and Standardization.
Extracellular Vesicle Flow Cytometry Analysis and Standardization.
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DOI：
10.3389/fcell.2017.00078
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发表时间：
2017
影响因子：
5.5
通讯作者：
Englyst NA
中科院分区：
文献类型：
--
作者：
Welsh JA;Holloway JA;Wilkinson JS;Englyst NA
细胞外囊泡（EVs）一词描述了来源于细胞的膜状囊泡，直径从30到1000纳米不等，大多数被认为在100-150纳米之间。由于其直径小，组成复杂多变，传统技术难以准确计数和表型ev。目前，与其他现有技术相比，使用高分辨率流式细胞术表征EV是最有前途的方法，因为它是一种高通量、单颗粒、多参数分析技术，能够分析大范围的颗粒直径。虽然高分辨率流式细胞术有望检测全EV直径范围，但不同流式细胞仪之间光散射和荧光数据的标准化仍然是一个问题。本文就高分辨率流式细胞术的研究进展以及EV散射和荧光标准化的未来发展方向作一综述。因此，研究小组和仪器之间缺乏标准化和可重复性，阻碍了电动汽车作为诊断生物标志物和治疗方法的验证。
The term extracellular vesicles (EVs) describes membranous vesicles derived from cells, ranging in diameter from 30 to 1,000 nm with the majority thought to be in the region of 100–150 nm. Due to their small diameter and complex and variable composition, conventional techniques have struggled to accurately count and phenotype EVs. Currently, EV characterization using high-resolution flow cytometry is the most promising method when compared to other currently available techniques, due to it being a high-throughput, single particle, multi-parameter analysis technique capable of analyzing a large range of particle diameters. Whilst high resolution flow cytometry promises detection of the full EV diameter range, standardization of light scattering and fluorescence data between different flow cytometers remains an problem. In this mini review, we will discuss the advances in high-resolution flow cytometry development and future direction of EV scatter and fluorescence standardization. Standardization and therefore reproducibility between research groups and instrumentation is lacking, hindering the validation of EVs use as diagnostic biomarkers and therapeutics.