Three-dimensional morphology and gene expression mapping for the Drosophila blastoderm.
Three-dimensional morphology and gene expression mapping for the Drosophila blastoderm.
复制标题
果蝇胚盘的三维形态和基因表达图谱。
DOI：
10.1101/pdb.top067843
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发表时间：
2012
影响因子：
--
通讯作者：
Knowles,DavidW
中科院分区：
文献类型：
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作者：
Knowles,DavidW
要正确认识动物的转录网络，就必须对转录因子及其靶点的时空表达模式有充分的定量认识。胚胎染色后的视觉检查揭示了基因的模式，显示了细胞间表达水平的复杂变化。利用我们目前对动物基因组基本生物学及其转录调控网络组成部分的丰富知识，结合光学显微镜、计算机、图像和视觉分析等现有技术，我们应该能够以细胞分辨率捕获整个动物的转录网络（所有因素和靶标）的定量三维（3D）信息。也应该有可能将这些数据整合到一个单一的可计算分析的数据库中——一个图谱——这可能是揭示控制调控基因网络的新生物学的基础。本文描述了实现这些目标的进展，重点是果蝇。它描述了一套高通量的方法，用于在整个动物的细胞分辨率上创建基因表达和形态的第一个定量3D描述，并且它展示了一些通过定量图谱揭示的新生物学。
To properly understand the transcriptional network of animals, we must have full quantitative comprehension of the spatial and temporal expression patterns of transcription factors and their targets. Visual inspection of embryos stained to reveal the patterns of genes shows levels of expression that change from cell to cell in a complex manner. With our current wealth of knowledge regarding the basic biology of animal genomes and the components of their transcriptional regulatory networks, combined with current technologies in optical microscopy, computing, and image and vision analysis, we should be able to capture quantitative, three-dimensional (3D) information about the transcriptional network (all factors and targets) for an entire animal at cellular resolution. It should also be possible to assemble these data into a single computationally analyzable database—an atlas—that could be the basis for uncovering new biology governing regulatory gene networks. This article describes progress toward realizing these goals, with the focus on Drosophila melanogaster. It describes a suite of high-throughput methods that have been used to create the first quantitative 3D description of gene expression and morphology at cellular resolution in a whole animal, and it presents some of the new biology that has been revealed by this quantitative atlas.