Genetic polymorphisms in the MMP-1 and MMP-3 gene may contribute to chronic periodontitis in a Brazilian population
Genetic polymorphisms in the MMP-1 and MMP-3 gene may contribute to chronic periodontitis in a Brazilian population
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DOI：
10.1111/j.1600-051x.2006.00979.x
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发表时间：
2006-10-01
影响因子：
6.7
通讯作者：
de Souza, Ana Paula
中科院分区：
文献类型：
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作者：
Astolfi, Claudia Maria;Shinohara, Andre Luis;de Souza, Ana Paula
目的：基质金属蛋白酶-1和-3(MMP1，MMP3)是降解细胞外基质大分子的蛋白酶。这些酶在破坏牙周组织的过程中起着重要的作用。在基质金属蛋白酶-1和基质金属蛋白酶-3基因的启动子区域存在遗传多态性。本研究的目的是在巴西人群中调查这些基因变异与慢性牙周炎之间的关系。材料和方法：114名非吸烟者(n=114)表现为临床附着丧失<gt；=5 mm。对照受试者(n=109)不应出现牙周炎的临床信号。应用聚合酶链式反应-限制性片段长度多态性分析方法对基质金属蛋白酶-1(-1607 1G/2G，-519 A/G)和基质金属蛋白酶-3(-1612 5A/6A)基因启动子进行了基因分型。结果：在正常牙周组和牙周炎组中，-1607 1G/2G和-519 A/G变异的分布无显著差异(p>；0.05)。然而，MMP3基因-16125A/6A多态的基因型频率分布显示，5A/5A型在牙周炎组中的频率显著高于对照组(p=0.008)。当只携带一个5A等位基因时，在5A/6A基因型中没有观察到同样的情况。我们还观察到在牙周炎组中，MMP1/MMP3单倍型(2G/5A)的频率有增加的趋势(p=0.08)。结论：在巴西人群中，未发现MMP1基因多态与牙周炎的易感性相关，而MMP3基因多态可能参与了牙周炎时牙周组织的破坏。
Objectives: Matrix metalloproteinase-1 and -3 (MMP-1, MMP-3) represent proteinases that degrade macromolecules of the extracellular matrix. These enzymes play a fundamental role during destruction of periodontal tissues. Genetic polymorphisms were characterized in the promoter region of the MMP-1 and MMP-3 genes. The aim of this study was to investigate the relationship between these genetic variations with chronic periodontitis in a Brazilian population.Material and Methods: Non-smoking subjects (n=114) exhibiting sites >= 5 mm clinical attachment loss were recruited for study. Control subjects (n=109) should not exhibit clinical signals of periodontitis. MMP-1 (-1607 1G/2G, -519 A/G) and MMP-3 (-1612 5A/6A) gene promoter polymorphisms were genotyped using PCR-RFLP methods.Results: Analysis of polymorphisms showed no differences in distribution of the -1607 1G/2G and -519 A/G variants in the MMP-1 gene between the healthy and periodontitis group (p > 0.05). However, the distribution of genotype frequencies of the -1612 5A/6A polymorphism in the MMP-3 gene showed that the 5A/5A genotype was significantly more frequent in the periodontitis group (p=0.008). The same was not observed in the 5A/6A genotype once only one 5A allele is carried. We also observed a trend to increase the frequency of the MMP-1/MMP-3 haplotype (2G/5A) in the periodontitis group (p=0.08).Conclusion: On the basis of the results, no significant association is found for the MMP-1 polymorphisms with susceptibility of periodontitis, while the MMP-3 gene polymorphism may contribute to periodontal tissue destruction during periodontitis in Brazilian subjects.