Intracellular trafficking of silicon particles and logic-embedded vectors
Intracellular trafficking of silicon particles and logic-embedded vectors
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DOI：
10.1039/c0nr00227e
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发表时间：
2010-01-01
期刊：
影响因子：
6.7
通讯作者：
Serda, Rita E.
中科院分区：
文献类型：
--
作者：
Ferrati, Silvia;Mack, Aaron;Serda, Rita E.
介孔硅颗粒作为第二阶段纳米颗粒和更高阶颗粒或治疗药物的载体，在药物输送和成像应用中显示出巨大的前景。颗粒几何形状、表面化学和孔隙度的调节使硅颗粒能够针对特定应用进行优化，例如血管靶向和避免在药物注射部位和最终目的地之间常见的生物屏障。在这项研究中，研究了未负载的载体硅颗粒和负载二次氧化铁纳米颗粒的载体颗粒在细胞内的运输。在细胞摄取后，膜包裹的硅颗粒通过微管驱动机制迁移到细胞的核周区域。颗粒的表面电荷、形状（球形和半球形）和大小（1.6和3.2 μ m）没有改变迁移速率。吞噬体的成熟与酸性的增加和后期内体和溶酶体标记物的获得有关。细胞摄取氧化铁纳米颗粒负载的硅颗粒导致颗粒的分类和运输到独特的目的地。硅载体仍然局限于吞噬体中，而第二阶段氧化铁纳米颗粒被分类成多泡体，这些多泡体从吞噬体中分离到新的膜结合室中。铁从细胞中释放可能代表载氧化铁纳米颗粒囊泡的胞吐作用。这些结果强化了多功能纳米载体的概念，其中不同的颗粒能够执行特定的任务，以便将单一或多组分的有效载荷传递到特定的亚细胞区室。
Mesoporous silicon particles show great promise for use in drug delivery and imaging applications as carriers for second-stage nanoparticles and higher order particles or therapeutics. Modulation of particle geometry, surface chemistry, and porosity allows silicon particles to be optimized for specific applications such as vascular targeting and avoidance of biological barriers commonly found between the site of drug injection and the final destination. In this study, the intracellular trafficking of unloaded carrier silicon particles and carrier particles loaded with secondary iron oxide nanoparticles was investigated. Following cellular uptake, membrane-encapsulated silicon particles migrated to the perinuclear region of the cell by a microtubule-driven mechanism. Surface charge, shape (spherical and hemispherical) and size (1.6 and 3.2 mu m) of the particle did not alter the rate of migration. Maturation of the phagosome was associated with an increase in acidity and acquisition of markers of late endosomes and lysosomes. Cellular uptake of iron oxide nanoparticle-loaded silicon particles resulted in sorting of the particles and trafficking to unique destinations. The silicon carriers remained localized in phagosomes, while the second stage iron oxide nanoparticles were sorted into multi-vesicular bodies that dissociated from the phagosome into novel membrane-bound compartments. Release of iron from the cells may represent exocytosis of iron oxide nanoparticle-loaded vesicles. These results reinforce the concept of multi-functional nanocarriers, in which different particles are able to perform specific tasks, in order to deliver single- or multi-component payloads to specific sub-cellular compartments.