New insight into cofactor-free oxygenation from combined experimental and computational approaches
New insight into cofactor-free oxygenation from combined experimental and computational approaches
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DOI：
10.1016/j.sbi.2016.06.015
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发表时间：
2016-12-01
影响因子：
6.8
通讯作者：
Steiner, Roberto A.
中科院分区：
文献类型：
--
作者：
Bui, Soi;Steiner, Roberto A.
尽管分子氧(O-2)是一种潜在的强氧化剂，但它与有机分子的反应性通常很差。这在很大程度上是由于量子化学的原因，因为O-2在其基态是双自由基(O-3(2))，而常见的有机底物处于单重态。因此，以O-2为反应物的催化通常是由含有氧化还原金属和/或有机辅助因子的酶来介导的。因此，从基本的观点来看，辅因子非依赖性加氧酶(和加氧酶)是很有趣的酶。这篇综述着眼于最近在理解这类耐人寻味的生物催化剂方面取得的进展，强调了涉及结构生物学、光谱学和理论方法的跨学科方法的力量。
Molecular oxygen (O-2), in spite being a potentially strong oxidant, typically displays very poor reactivity with organic molecules. This is largely due to quantum chemical reasons as O-2 in its ground state is a diradical (O-3(2)) whilst common organic substrates are in a singlet state. For this reason catalysis involving O-2 as a reactant is typically mediated by enzymes containing redox metal and/or organic co-factors. Cofactor independent oxygenases (and oxidases) are therefore intriguing enzymes from a fundamental viewpoint. This review looks at recent advances that have been made in understanding of this class of intriguing biocatalysts highlighting the power of an inter-disciplinary approach involving structural biology, spectroscopy and theoretical methods.