EXPRESSION OF TGF-BETA AND PROCOLLAGEN TYPE-I AND TYPE-III IN HUMAN GASTRIC CARCINOMAS
EXPRESSION OF TGF-BETA AND PROCOLLAGEN TYPE-I AND TYPE-III IN HUMAN GASTRIC CARCINOMAS
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DOI：
10.1002/ijc.2910440303
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发表时间：
1989-09-15
影响因子：
6.4
通讯作者：
TAHARA, E
中科院分区：
文献类型：
--
作者：
YOSHIDA, K;YOKOZAKI, H;TAHARA, E
为了阐明硬癌和非硬癌胃癌之间的差异，我们检测了TGF-β，7种胃癌细胞系和37种胃癌组织中的I型和III型前胶原，并且还检测了TGF-β的作用。对TMK-I细胞前胶原mRNA表达的影响。TGF-.beta.在体内和体外检测的所有肿瘤中均检测到mRNA。有趣的是，10例硬化性胃癌中有9例（90%）显示出更高水平的TGF-β。21例高分化腺癌中有8例（38%）具有较高的TGF-β mRNA。mRNA水平高于正常组织。在前胶原mRNA方面，大多数人胃癌细胞株表达I型前胶原mRNA，MKN-1表达III型前胶原mRNA。此外，外源性TGF-β增加了TMK-I细胞中I型前胶原mRNA的积累。手术切除的肿瘤组织中，前胶原mRNA的表达大多高于正常组织。这些结果表明，TGF-β癌细胞分泌的胶原不仅刺激成纤维细胞合成胶原，而且刺激癌细胞自身合成胶原，导致硬化性胃癌的弥漫性纤维化。
To elucidate the difference between scirrhous and non-scirrhous gastric carcinomas, we examined the expressions of TGF-.beta., procollagen type I and type III in 7 gastric carcinoma cell lines and 37 gastric carcinoma tissues, and also examined the effect of TGF-.beta. on the expression of procollagen mRNA by TMK-I cells. TGF-.beta. mRNA was detected in all the tumors examined in vivo and in vitro. Interestingly, 9 (90%) of 10 scirrhous gastric carcinomas revealed higher levels of TGF-.beta. mRNA than normal tissues, while 8 (38%) of 21 well-differentiated adenocarcinomas had higher TGF-.beta. mRNA levels than normal tissues. As for procollagen mRNA, most of the human gastric carcinoma cell lines expressed type-I procollagen mRNA and MKN-I expressed type-III procollagen mRNA. Furthermore, procollagen type-I mRNA accumulation in TMK-I cells was increased by exogenous TGF-.beta.. Most of the tumor tissues from surgical specimens expressed higher procollagen mRNA than normal tissues. These results indicate that TGF-.beta. produced by carcinoma cells might stimulate collagen synthesis not only by fibroblasts but also by carcinoma cells themselves, leading to diffuse fibrosis of scirrhous gastric carcinomas.