In vitro earthworm Lumbricus terrestris coelomocyte assay for use in terrestrial toxicity identification evaluation
In vitro earthworm Lumbricus terrestris coelomocyte assay for use in terrestrial toxicity identification evaluation
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DOI：
10.1007/s001289900910
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发表时间：
1999-05-01
影响因子：
2.7
通讯作者：
Giggleman, MA
中科院分区：
文献类型：
--
作者：
Burch, SW;Fitzpatrick, LC;Giggleman, MA
描述了一种体外吞噬细胞和细胞活力测定，其允许直接测定。terrestriscoelomocyte暴露于小体积和低浓度的毒素。结果包括体腔细胞活力和吞噬作用对无机（CuSO 4）和有机（Aroclor 1254 [氯化1，1 ′-联苯]）化学品的暴露反应敏感性评估。比较结果的腔肠菌暴露在体外和在vivouing最低可观察到的影响水平（LOEL）的化学品暴露浓度。基于LOEL，体外活性和吞噬作用的绝对灵敏度< 1.0 μg Cu 2+，而吞噬作用对氯代1，1 ′-联苯的灵敏度似乎比腔肠菌活性高200倍，体外活性和吞噬作用对Cu 2+和氯代1，1 ′-联苯的灵敏度分别比体内活性高≥10.7× 103和8.94× 106倍。 活性和吞噬作用的影响，通过增加浓度的毒物。
Anin vitrophagocytic and cell viability assay is described that allows for directL. terrestriscoelomocyte exposure to small volumes and low concentrations of toxins. The results are included of assessments of the exposure response sensitivity of coelomocyte viability and phagocytosis to inorganic (CuSO4) and organic (Aroclor 1254 [chlorinated 1,1′-biphenyl]) chemicals. Results of comparisons of coelomycetes exposedin vitroandin vivousing lowest observable effects levels (LOELs) of chemical exposure concentration are given. Based on LOELs, absolute sensitivity ofin vitroviability and phagocytosis assays was < 1.0 µg Cu2+, whilst phagocytosis appeared to be 200 × more sensitive than coelomycete viability to chlorinated 1,1′-biphenyl.In vitroassays were ≥10.7 ×103and 8.94 ×106times more sensitive thanin vivoassays for Cu2+and chlorinated 1,1′-biphenyl, respectively. Both viability and phagocytosis were affected by increasing concentrations of toxicants.