Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability
Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability
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DOI：
10.1007/s11103-004-5293-6
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发表时间：
2004-12-01
影响因子：
5.1
通讯作者：
Grabau, EA
中科院分区：
文献类型：
--
作者：
Chiera, JM;Finer, JJ;Grabau, EA
利用转基因技术改变大豆种子的植酸含量，在种子发育过程中表达大豆植酸酶基因(GmPhy)，以降解积累的植酸(IP6)。利用种子特异的β-伴球蛋白启动子(α‘亚基)调控的大豆植酸酶基因的表达载体转化大豆胚性培养物。对来自4个独立转基因系的植株进行了转基因整合和种子IP6水平的分析。高效液相色谱检测表明，与对照‘杰克’大豆相比，转基因大豆种子中IP6水平降低了12.6到24.8。一个低拷贝转化子被繁殖到T-4代，并对种子发育过程中植酸酶的表达和酶活性进行了更详细的检测。植酸酶mRNA的表达和植酸酶活性在种子发育过程中增加，这与胚胎特异性启动子的使用是一致的。异位植酸酶在种子发育过程中的表达为降低大豆种子中植酸含量提供了一种有效的策略。
A transgenic approach was used to alter soybean seed phytate content by expressing a soybean phytase gene (GmPhy) during seed development to degrade accumulating phytic acid (IP6). An expression vector containing the soybean phytase cDNA controlled by the seed-specific beta-conglycinin promoter (alpha'-subunit) was used to transform embryogenic soybean cultures. Plants from four independent transgenic lines were analyzed for transgene integration and seed IP6 levels. The reduction in IP6 levels in transgenic seeds compared to control 'Jack' soybeans ranged from 12.6 to 24.8 as determined by HPLC. A low copy transformant was propagated to the T-4 generation and examined in more detail for phytase expression and enzyme activity during seed development. Expression of phytase mRNA and phytase activity increased during seed development, consistent with the use of an embryo-specific promoter. Ectopic phytase expression during seed development offers potential as an effective strategy for reducing phytate content in soybean seed.