Isolation and Identification of Potential Phosphate Solubilizing Bacteria from the Rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh
Isolation and Identification of Potential Phosphate Solubilizing Bacteria from the Rhizoplane of Oryza sativa L. cv. BR29 of Bangladesh
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DOI：
10.1515/znc-2007-1-218
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发表时间：
2007-02
期刊：
影响因子：
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通讯作者：
M. Islam;A. Deora;Y. Hashidoko;Atiqur Rahman;Toshiaki Ito;S. Tahara
中科院分区：
文献类型：
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作者：
M. Islam;A. Deora;Y. Hashidoko;Atiqur Rahman;Toshiaki Ito;S. Tahara
从水稻品种根际分离到30株细菌。BR29在孟加拉的Mymensingh种植，并从BR29的表面灭菌种子中获得幼苗。经筛选，6个菌株在琼脂平板和发酵液中均表现出不同程度的解磷活性，采用国家植物研究所的磷酸盐培养基。根据表型和16S rRNA基因测序数据，将分离的菌株鉴定为不动杆菌属。BR-12，克雷伯氏菌。BR-15，不动杆菌属。BR-25，肠杆菌属。BR-26，微杆菌属。BRS-1和假单胞菌属。BRS-2。BR-25的增磷活性最高，其次是BR-15。在pH值为5和7时，它们在液体中生长迅速，而在pH值为3时，几乎没有生长。随着细菌的生长，培养液的pH值下降，这表明它们可能分泌有机酸来溶解不溶性磷。对接种BR-25和BR-15的种子萌发的两周大水稻幼苗的扫描电子显微镜分析表明，根面密集定植，可能是细菌细胞上的菌毛。
A total of 30 bacteria were isolated from the rhizoplane of rice cv. BR29 cultivated in Mymensingh, Bangladesh and from the seedlings obtained from surface-sterilized seeds of BR29. Upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using National Botanical Research Institute’s phosphate medium. The bacterial isolates were identified based on their phenotypic and 16S rRNA genes sequencing data as Acinetobacter sp. BR-12, Klebsiella sp. BR-15, Acinetobacter sp. BR-25, Enterobacter sp. BR-26, Microbacterium sp. BRS-1 and Pseudomonas sp. BRS-2. The BR-25 exhibited highest phosphate solubilizing activity followed by BR-15. They grew rapidly in the liquid medium at pH 5 and 7 but almost no growth occurred at pH 3. The pH value of the culture medium was decreased with bacterial growth suggesting that they might secrete organic acids to solubilize insoluble phosphorus. Scanning electron microscope analysis of two-week-old rice seedlings germinated from seeds previously inoculated with BR-25 and BR-15 revealed dense colonization at the root surfaces presumably using fimbriae on the bacterial cells.