Cellular changes in the hamster testicular interstitium with ageing and after exposure to short photoperiod
Cellular changes in the hamster testicular interstitium with ageing and after exposure to short photoperiod
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DOI：
10.1071/rd14117
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发表时间：
2016-01-01
影响因子：
1.9
通讯作者：
Pastor, Luis Miguel
中科院分区：
文献类型：
--
作者：
Beltran-Frutos, E.;Seco-Rovira, V.;Pastor, Luis Miguel
本研究的目的是评估细胞的变化，发生在仓鼠的睾丸退化在两个非常不同的生理情况下，涉及睾丸退化：老化和暴露于短的光周期。这些动物被分为一个“年龄组”，其中有三个亚组-年轻，成年和老年动物-和一个“回归组”，其中动物受到短光周期。光镜和电镜下观察睾丸结节。组织化学研究间质细胞的增殖，末端脱氧核苷酸转移酶（TdT）介导的dUTP原位缺口末端标记（TUNEL+）和睾酮合成活性。我们鉴定了两种类型的间质细胞：A型细胞显示正常形态，而B型细胞出现坏死。随着年龄的增长，周细胞增殖减少，但有TUNEL阳性的间质细胞的指数没有变化。在消退组中，周细胞增殖更大，TUNEL阳性细胞中未观察到。衰老过程中睾丸间质细胞超微结构变化不大，间质细胞坏死。与此相反，退化组中观察到间质细胞的超微结构退化，无坏死。总之，中仓鼠的睾丸睾丸细胞在两组（年龄和回归）中表现出不同的细胞变化，可能是由于老化的不可逆性和短光周期引起的变化的可逆性。
The aim of this study was to evaluate the cellular changes that occur in the hamster testicular interstitium in two very different physiological situations involving testicular involution: ageing and exposure to a short photoperiod. The animals were divided into an 'age group' with three subgroups - young, adult and old animals - and a 'regressed group' with animals subjected to a short photoperiod. The testicular interstitium was characterised by light and electron microscopy. Interstitial cells were studied histochemically with regard to their proliferation, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP in situ nick end labelling (TUNEL+) and testosterone synthetic activity. We identified two types of Leydig cell: Type A cells showed a normal morphology, while Type B cells appeared necrotic. With ageing, pericyte proliferation decreased but there was no variation in the index of TUNEL-positive Leydig cells. In the regressed group, pericyte proliferation was greater and TUNEL-positive cells were not observed in the interstitium. The testicular interstitium suffered few ultrastructural changes during ageing and necrotic Leydig cells were observed. In contrast, an ultrastructural involution of Leydig cells with no necrosis was observed in the regressed group. In conclusion, the testicular interstitium of Mesocricetus auratus showed different cellular changes in the two groups (age and regressed), probably due to the irreversible nature of ageing and the reversible character of changes induced by short photoperiod.