Novel antimicrobial activities of a peptide derived from a Japanese soybean fermented food, Natto, against Streptococcus pneumoniae and Bacillus subtilis group strains.
Novel antimicrobial activities of a peptide derived from a Japanese soybean fermented food, Natto, against Streptococcus pneumoniae and Bacillus subtilis group strains.
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DOI：
10.1186/s13568-017-0430-1
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发表时间：
2017-12
期刊：
影响因子：
3.7
通讯作者：
Yokota SI
中科院分区：
文献类型：
--
作者：
Kitagawa M;Shiraishi T;Yamamoto S;Kutomi R;Ohkoshi Y;Sato T;Wakui H;Itoh H;Miyamoto A;Yokota SI
我们最近从日本传统发酵食品纳豆中分离到一种杀肿瘤肽。在本研究中，检查纳豆肽的抗微生物活性。该肽由45个氨基酸残基组成，推测其结构为富含α-螺旋。该菌株仅对肺炎链球菌和枯草芽孢杆菌群（B.枯草芽孢杆菌、短小芽孢杆菌和地衣芽孢杆菌）。观察到除链球菌外的链球菌种的抗菌活性较低。肺炎。肽的抗微生物活性需要溶血产物或氯化血红素。纳豆肽破坏了B的细胞膜。枯草杆菌。另一方面，在S.在纳豆肽处理的早期阶段，使用天然双球菌的肺炎双球菌培养细胞;随后细胞被快速裂解。这表明纳豆肽显示了一种新的窄谱杀菌活性，并抑制了S.肺炎。
We recently isolated a tumoricidal peptide from Natto, a Japanese traditional fermented food. In the present study, antimicrobial activity of the Natto peptide was examined. The peptide consisted of 45 amino acid residues, and its structure was predicted to be rich in α-helix. It excreted antimicrobial activity only against Streptococcus pneumoniae and Bacillus subtilis group (B. subtilis, Bacillus pumilus, and Bacillus licheniformis). Lesser antimicrobial activity was observed for Streptococcus species other than S. pneumoniae. Hemolysate or hemin was required for the antimicrobial activity of the peptide. The Natto peptide damages the cell membrane of B. subtilis. On the other hand, chain morphology was induced in S. pneumoniae, which is naturally diplococcus, during the early phases of the Natto peptide treatment; following that the cells were rapidly lysed. This suggested that the Natto peptide displayed a novel narrow spectrum of bactericidal activity and inhibited cell separation during cell division of S. pneumoniae.