Changes in poly[d(T-G).d(C-A)] chirality due to Hg(II)-binding: circular dichroism (CD) studies.
Changes in poly[d(T-G).d(C-A)] chirality due to Hg(II)-binding: circular dichroism (CD) studies.
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Hg(II) 结合引起的聚[d(T-G).d(C-A)]手性变化：圆二色性 (CD) 研究。
DOI：
10.1016/0162-0134(91)84066-i
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发表时间：
1991
影响因子：
3.9
通讯作者：
Cruikshank,MK
中科院分区：
文献类型：
--
作者：
Gruenwedel,DW;Cruikshank,MK
用紫外圆二色性（CD）光谱研究了聚[d（T-G）·d(C-A)]在0.1 M NaClO4, 5 mM羧酸缓冲液，pH 6.9中的构象。在Hg(ClO4)2的存在下，多核苷酸的构象以高度合作的方式发生变化，这反映在其热带特征上。CD将信号转变首先从B转变为修饰的B态（B*），或转变为称为X的非B结构，最后转变为一种可能是z的形式。在适当的络合剂（如氰化钠）的帮助下去除汞后，这种转变是完全可逆的，这表明汞在任何程度上都不会破坏沃森-克里克氢键。
The conformation of poly[d(T-G)·d(C-A)] in aqueous solution (0.1 M NaClO4, 5 mM cacodylic acid buffer, pH 6.9) was studied by circular dichroism (CD) spectroscopy in the ultraviolet. The conformation of the polynucleotide, as reflected by its chiroptical signature, changes in a highly cooperative fashion in the presence of Hg(ClO4)2. The CD changes signal transitions first from the B to a modified B-state (B*), or to a non-B structure termed X, and finally to a form that is presumably Z. The alterations are totally reversible subsequent to the removal of mercury with the help of a suitable complexing agent such as sodium cyanide, indicating that mercuration does not disrupt Watson-Crick hydrogen bonding to any extent.