TaVRT-2, a member of the StMADS-11 clade of flowering repressors, is regulated by vernalization and photoperiod in wheat
TaVRT-2, a member of the StMADS-11 clade of flowering repressors, is regulated by vernalization and photoperiod in wheat
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DOI：
10.1104/pp.105.061762
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发表时间：
2005-08-01
期刊：
影响因子：
7.4
通讯作者：
Sarhan, F
中科院分区：
文献类型：
--
作者：
Kane, NA;Danyluk, J;Sarhan, F
冬季谷物繁殖阶段的开始在冬季被推迟，直到有利的生长条件在春季恢复。这种延迟是由低温通过春化过程调节的。环境因子与植物成花转变相互作用的分子和遗传基础尚不清楚。然而，最近发现的小麦（Triticum aestivum L.）TaVRT-1基因为破解谷物开花时间调控的分子基础提供了机会。在这里，我们描述了另一个可能参与小麦开花途径的基因TaVRT-2的特征。分子和系统发育分析表明，该基因编码MADS-box转录因子家族的一个成员，该家族在一些物种中属于抑制开花的分支。TaVRT-2在近等基因系和不同基因型中对春化和光周期的响应具有自然变异，其表达谱与花的转变密切相关。它的表达在营养期冬季基因型中上调，在短日照期光敏基因型中上调，并在春化过程中被抑制到允许向生殖期过渡的水平。蛋白-蛋白相互作用研究表明，TaVRT-2与小麦两个重要春化基因（TaVRT-1/VRN-1和VRN-2）编码的蛋白相互作用。这些结果支持了TaVRT-2是小麦花转化的推定抑制因子的假设。
The initiation of the reproductive phase in winter cereals is delayed during winter until favorable growth conditions resume in the spring. This delay is modulated by low temperature through the process of vernalization. The molecular and genetic bases of the interaction between environmental factors and the floral transition in these species are still unknown. However, the recent identification of the wheat ( Triticum aestivum L.) TaVRT-1 gene provides an opportunity to decipher the molecular basis of the flowering-time regulation in cereals. Here, we describe the characterization of another gene, named TaVRT-2, possibly involved in the flowering pathway in wheat. Molecular and phylogenetic analyses indicate that the gene encodes a member of the MADS-box transcription factor family that belongs to a clade responsible for flowering repression in several species. Expression profiling of TaVRT-2 in near-isogenic lines and different genotypes with natural variation in their response to vernalization and photoperiod showed a strong relationship with floral transition. Its expression is up-regulated in the winter genotypes during the vegetative phase and in photoperiod-sensitive genotypes during short days, and is repressed by vernalization to a level that allows the transition to the reproductive phase. Protein-protein interaction studies revealed that TaVRT-2 interacts with proteins encoded by two important vernalization genes ( TaVRT-1/VRN-1 and VRN-2) in wheat. These results support the hypothesis that TaVRT-2 is a putative repressor of the floral transition in wheat.