Detection and validation of QTLs associated with seed longevity in rice (Oryza sativa L.)
Detection and validation of QTLs associated with seed longevity in rice (Oryza sativa L.)
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与水稻种子寿命相关的 QTL 的检测和验证 (Oryza sativa L.)
DOI：
10.1111/pbr.12611
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发表时间：
2018-08-01
期刊：
影响因子：
2
通讯作者：
Li, Xiaoxiang
中科院分区：
文献类型：
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作者：
Liu, Wenqiang;Pan, Xiaowu;Li, Xiaoxiang
水稻种子寿命是决定种子生产和种质保存的重要因素。以香743与凯蒂杂交的172个重组自交系（RIL）群体为材料，在环境条件下对种子贮藏18个月和30个月后的种子寿命（SL）进行了定量性状位点（qtl）定位。两个推测的qtl qSL-2和qSL-8分别位于2号和8号染色体上。qSL-2是湘743等位基因的一个等位基因，可以增加种子寿命。qSL-8是从凯蒂的等位基因中提取的一个新的QTL，可以提高种子的寿命。qSL-8分别解释了贮藏18个月和30个月后表型变异的15.29%和17.35%。此外，qSL-8在RIL群体中选择的剩余杂合系（RHL）自花授粉形成的二级群体中得到了验证，该群体解释了25.93%的表型贡献。这些结果为qSL-8的图谱克隆提供了机会。此外，qSL-8可能是通过标记辅助选择（MAS）提高水稻种子寿命的靶点。
Seed longevity in rice is a major determinant in seed production and germplasm preservation. In this paper, a recombinant inbred line (RIL) population consisting of 172 lines derived from the cross between Xiang743 and Katy' was used to map quantitative trait loci (QTLs) for seed longevity (SL) after seed storage for 18 and 30months under ambient conditions. Two putative QTLs, qSL-2 and qSL-8, were detected and located on chromosomes 2 and 8, respectively. qSL-2 is an allele from Xiang743 allele and increases seed longevity. qSL-8 was a novel QTL from Katy' allele and increases seed longevity. qSL-8 explained 15.29% and 17.35% of the phenotypic variance after seed storage for 18 and 30months, respectively. Furthermore, qSL-8 was validated in a secondary population developed by self-pollination of a residual heterozygous line (RHL) selected from the RIL population, which explained 25.93% of the phenotypic contribution. These results provide an opportunity for map-based cloning of qSL-8. Furthermore, qSL-8 may be a target for improving seed longevity by marker-assisted selection (MAS) in rice.